ESTABLISHMENT OF IFA TECHNIQUE FOR DETECTING ANCA

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SAEIDI M.; BARADARAN H.; HATEF M.A.

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Paper Information

Journal: JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES Year:2002 | Volume:3 | Issue:8 Page(s): 12-17

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Information Journal Paper

Title

ESTABLISHMENT OF IFA TECHNIQUE FOR DETECTING ANCA

Author(s)

SAEIDI M. | BARADARAN H. | HATEF M.A. | Issue Writer Certificate

Keywords

ANCAQ4

IFAQ4

Abstract

ANCA are classes of Antibodies or in reality Autoantibodies usually produce in conditions that degradation of neutrophiles occur. This is led to vascular damage. They are more common in Rheumatic diseases such as Rheumatoid Arthritis (RA), systemic lupus Erythematus (SLE) and vasculitic diseases especially Wegners" granulomatosis (with specificityand sensitivity as high as 95%). We decided to design IFA-ANCA because this assay has high sensitivity and in with comparision other techniques such as EL/SA & RIA is more economic and simple. On the other hand, screening test for determining ANCA is immunofluorescent assay.ANCA test establishment contains: Isolation of neutrophiles, fixation with ethanol and formalin, improvement of method and quality control by using qf indirect immunoflourescence assay (IFA), two staining pattern are observed. Cytoplasmic (C-ANCA) or classic and prinuclear (P-ANCA) pattern. Established ANCA test by inter assay method had 95% reproducibility and 91 % precision.

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APA: Copy

SAEIDI, M., BARADARAN, H., & HATEF, M.A.. (2002). ESTABLISHMENT OF IFA TECHNIQUE FOR DETECTING ANCA. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 3(8), 12-17. SID. https://sid.ir/paper/79025/en

Vancouver: Copy

SAEIDI M., BARADARAN H., HATEF M.A.. ESTABLISHMENT OF IFA TECHNIQUE FOR DETECTING ANCA. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2002;3(8):12-17. Available from: https://sid.ir/paper/79025/en

IEEE: Copy

M. SAEIDI, H. BARADARAN, and M.A. HATEF, “ESTABLISHMENT OF IFA TECHNIQUE FOR DETECTING ANCA,” JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, vol. 3, no. 8, pp. 12–17, 2002, [Online]. Available: https://sid.ir/paper/79025/en

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