HETEROLOGOUS EXPRESSION OF SALMON CALCITONIN IN ESCHERICHIA COLI

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POURHASHEM Z.; ABBASIAN M.; SHAHBAZI M.; YAMCHI A.

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Journal Paper

Paper Information

Journal: JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES Year:2017 | Volume:19 | Issue:1 (61) Page(s): 77-82

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Title

HETEROLOGOUS EXPRESSION OF SALMON CALCITONIN IN ESCHERICHIA COLI

Author(s)

POURHASHEM Z. | ABBASIAN M. | SHAHBAZI M. | YAMCHI A. | Issue Writer Certificate

Keywords

CALCITONINQ2

THIOREDOXIN FUSION TAGQ1

OVER EXPRESSIONQ1

PEPTIDE HORMONEQ1

Abstract

Background and Objective: CALCITONIN is a small PEPTIDE HORMONE including 32 amino acids and 3.4 KD molecular weight which is produced by the parafollicular cells of the thyroid gland in respond to increasing calcium ions in serum. This peptide is used for adjuvant therapy of osteoporosis, Paget' s disease and hypercalcemic shock. In this study, the heterologuse expression of CALCITONIN was done in Escherichia coli.Methods: In this experimental study, the thioredoxin fusion partner was added to n-terminal of the Salmon calciton in order to increase its stability by the synthetic biology. The recombinant construct was transformed and over expressed into Escherichia coli BL21 (DE3) host cell.Results: SDS-PAGE analysis showed the OVER EXPRESSION of recombinant protein after IPTG induction.Conclusion: In this study, the construct including fused Salmon CALCITONIN gene with thioredoxin was cloned. The SDS-PAGE result showed the stable expression of fused CALCITONIN.

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APA: Copy

POURHASHEM, Z., ABBASIAN, M., SHAHBAZI, M., & YAMCHI, A.. (2017). HETEROLOGOUS EXPRESSION OF SALMON CALCITONIN IN ESCHERICHIA COLI. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 19(1 (61) ), 77-82. SID. https://sid.ir/paper/79400/en

Vancouver: Copy

POURHASHEM Z., ABBASIAN M., SHAHBAZI M., YAMCHI A.. HETEROLOGOUS EXPRESSION OF SALMON CALCITONIN IN ESCHERICHIA COLI. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;19(1 (61) ):77-82. Available from: https://sid.ir/paper/79400/en

IEEE: Copy

Z. POURHASHEM, M. ABBASIAN, M. SHAHBAZI, and A. YAMCHI, “HETEROLOGOUS EXPRESSION OF SALMON CALCITONIN IN ESCHERICHIA COLI,” JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, vol. 19, no. 1 (61) , pp. 77–82, 2017, [Online]. Available: https://sid.ir/paper/79400/en

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