IDENTIFICATION OF MORPHOLOGICAL AND MOLECULAR ASPERGILLUS SPECIES ISOLATED FROM PATIENTS BASED ON BETA-TUBULIN GENE SEQUENCING

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KHEIRKHAH M.; CHADEGANIPOUR M.; DEHGHAN P.; MOHAMMADI R.

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Journal: Yafteh Year:2017 | Volume:19 | Issue:1 (71) Page(s): 87-97

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Information Journal Paper

Title

IDENTIFICATION OF MORPHOLOGICAL AND MOLECULAR ASPERGILLUS SPECIES ISOLATED FROM PATIENTS BASED ON BETA-TUBULIN GENE SEQUENCING

Author(s)

KHEIRKHAH M. | CHADEGANIPOUR M. | DEHGHAN P. | MOHAMMADI R. | Issue Writer Certificate

Keywords

ASPERGILLUSQ2

BETA TUBULINQ1

SEQUENCINGQ2

Abstract

Background: ASPERGILLUS species are opportunistic pathogens among immunocompromised patients. In terms of pathogenesis and mycotoxin production, they are in great value. The aim of the this study was to evaluate of beta-tubulin gene for identification of clinicalASPERGILLUS species by PCR-sequencing method compared to morphological features of clinical isolates (such as conidial shape in direct microscopic examination, colony shape in culture, and physiological tests).Materials and Methods: In this study, 465 patients referred to the Shefa laboratory of Isfahan were evaluated. Morphological and molecular identification of clinical samples were performed using culture on sabouraud agar, malt extract agar, czapekdox agar, direct microscopy, and PCR-sequencing of BETA TUBULIN gene, respectively. Sequences were analyzed in comparison with gene bank data.Results: Thirty nine out of 465 suspected cases (8.4%) had aspergillosis. The most prevalent species were ASPERGILLUS flavus (56.4%), A. oryzae (20.5%), and A. fumigatus (10.2%), respectively. Fifty nine percent of patients were females and 49% were males.Conclusion: In comparison with phenotypic tests, SEQUENCING of beta-tubulin gene for identification of ASPERGILLUS species is at great value. Replacement of molecular techniques with conventional tests is recommended for precise identification of microorganism for better management of infection.

Cites

Toxigenicity and phylogeny of Aspergillus section Flavi in poultry feed in Iran

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APA: Copy

KHEIRKHAH, M., CHADEGANIPOUR, M., DEHGHAN, P., & MOHAMMADI, R.. (2017). IDENTIFICATION OF MORPHOLOGICAL AND MOLECULAR ASPERGILLUS SPECIES ISOLATED FROM PATIENTS BASED ON BETA-TUBULIN GENE SEQUENCING. YAFTEH, 19(1 (71)), 87-97. SID. https://sid.ir/paper/80011/en

Vancouver: Copy

KHEIRKHAH M., CHADEGANIPOUR M., DEHGHAN P., MOHAMMADI R.. IDENTIFICATION OF MORPHOLOGICAL AND MOLECULAR ASPERGILLUS SPECIES ISOLATED FROM PATIENTS BASED ON BETA-TUBULIN GENE SEQUENCING. YAFTEH[Internet]. 2017;19(1 (71)):87-97. Available from: https://sid.ir/paper/80011/en

IEEE: Copy

M. KHEIRKHAH, M. CHADEGANIPOUR, P. DEHGHAN, and R. MOHAMMADI, “IDENTIFICATION OF MORPHOLOGICAL AND MOLECULAR ASPERGILLUS SPECIES ISOLATED FROM PATIENTS BASED ON BETA-TUBULIN GENE SEQUENCING,” YAFTEH, vol. 19, no. 1 (71), pp. 87–97, 2017, [Online]. Available: https://sid.ir/paper/80011/en

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