APPLICATION OF SEMI-NESTED PCR RFLP FOR DETECTION AND GENOTYPING GBV-C AMONG HBV-INFECTED PATIENTS

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YAZDANI LALEH; RAVANSHAD MEHRDAD; KHANLARI ZAHRA; YAGHOOBI RAMIN

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Journal: Pathobiology Research Year:2012 | Volume:15 | Issue:1 Page(s): 81-91

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Title

APPLICATION OF SEMI-NESTED PCR RFLP FOR DETECTION AND GENOTYPING GBV-C AMONG HBV-INFECTED PATIENTS

Author(s)

YAZDANI LALEH | RAVANSHAD MEHRDAD | KHANLARI ZAHRA | YAGHOOBI RAMIN | Issue Writer Certificate

Keywords

GBV-CQ2

HBS-AG POSITIVEQ2

RFLPQ3

GENOTYPINGQ2

SEMI-NESTED PCRQ2

Abstract

Objective: GBV-C is a virus transmissible via blood transfusion and sexual routes. Because of the shared transmission routes in GBV-C and hepatitis B virus (HBV) we have attempted to detect GBV-C in HBsAg-positive patients using the more sensitive SEMI-NESTED PCR. We used restriction fragment length polymorphism (RFLP) to determine genotype.Methods: A total of 100 serum samples were collected from HBsAg-positive patients from 1389-1390. After designing specific primers and optimizing the SEMI-NESTED PCR, sequences of PCR products were analyzed by Neb Cutter software. Restriction enzyme sites were determined and suitable enzymes selected for RFLP.Results: SEMI-NESTED PCR shows acceptable sensitivity for the detection of GBV-C and its genotype determination. This technique is more affordable than other techniques.Conclusion: There appears to be a high rate of GBV-C infection among Iranian HBV patients. In this study, the majority of patients co-infected with GBV-C were of HBV genotype 2, which is similar to the pattern seen in the US and Europe.

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APA: Copy

YAZDANI, LALEH, RAVANSHAD, MEHRDAD, KHANLARI, ZAHRA, & YAGHOOBI, RAMIN. (2012). APPLICATION OF SEMI-NESTED PCR RFLP FOR DETECTION AND GENOTYPING GBV-C AMONG HBV-INFECTED PATIENTS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 15(1), 81-91. SID. https://sid.ir/paper/80984/en

Vancouver: Copy

YAZDANI LALEH, RAVANSHAD MEHRDAD, KHANLARI ZAHRA, YAGHOOBI RAMIN. APPLICATION OF SEMI-NESTED PCR RFLP FOR DETECTION AND GENOTYPING GBV-C AMONG HBV-INFECTED PATIENTS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2012;15(1):81-91. Available from: https://sid.ir/paper/80984/en

IEEE: Copy

LALEH YAZDANI, MEHRDAD RAVANSHAD, ZAHRA KHANLARI, and RAMIN YAGHOOBI, “APPLICATION OF SEMI-NESTED PCR RFLP FOR DETECTION AND GENOTYPING GBV-C AMONG HBV-INFECTED PATIENTS,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 15, no. 1, pp. 81–91, 2012, [Online]. Available: https://sid.ir/paper/80984/en

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