USE OF REAL-TIME PCR METHOD ON THE AMNIOCYTE SAMPLES FOR PRENATAL DIAGNOSIS OF DOWN SYNDROME

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHAHROKHI FAHIMEH; MOHADES ARDEBILI S.M.; FARDI AZAR Z.; KAMYAB AHMAD REZA; SHAMSIAN E.; HAYAT NOUSAEID M.; KAEINI MOGHADAM Z.; MAHDIAN R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2009 | Volume:12 | Issue:3 Page(s): 17-31

Download Full-Text

Persian Verion

View:

1,828

Download:

Cites:

Information Journal Paper

Title

USE OF REAL-TIME PCR METHOD ON THE AMNIOCYTE SAMPLES FOR PRENATAL DIAGNOSIS OF DOWN SYNDROME

Author(s)

Keywords

BORRELIA PERSICAQ2

ORNITHODOROS THOLOZANIQ2

TBRFQ2

PCRQ3

IRANQ3

Abstract

Objective: In this study, the possibility of prenatal diagnosis of Down syndrome with Real-Time PCR method was evaluated. In this context, optimization of a suitable method for purification of high quality DNA from amniotic fluid samples was also considered. Materials and Methods: Pregnant women who had the high risk of having babies with Down syndrome were selected according to the biochemical and sonographic data and referred to the amniocentesis center. The DNA of total 59 amniotic fluid samples were extracted with different methods including boiling method, salting out method, Procedures of DNA extraction from Blood and Cell Culture by DNPTM Kit (CinnaGen), Procedure of DNA extraction from cells by DNA Isolation Kit for cells and tissues (Roche), Procedure of DNA extraction from Tissue by MagNa Pure DNA Isolation kit (Roche), and QIAamp DNA Micro Kit (Qiagen). Then, the quality and quantity of the extracted DNA were evaluated by the NanoDrop® ND- 1000 spectrophotometer device. Real-Time PCR reaction using fluorescent dye SYBR Green I (Applied Biosystems, UK) was performed to specifically amplify DSCAM and DYRK1A2 genes and the reference gene (PMP22). Data analysis was performed using comparative cycle threshold method for the determination of the gene dosage and determining the number of copies of chromosome 21.Results: This study showed that DNA extracted from amniotic fluid samples using QIAamp DNA Micro Kit (Qiagen) has the desirable quantity and quality for Real-Time PCR. Specific proliferation of targets and reference genes was achieved and difference between normal and affected groups based on differences between their gene dosages was determined. Conclusion: Prenatal diagnosis of Down syndrome is feasible by the Real-Time PCR method using DNA samples from amniotic fluid cells extracted by QIAamp DNA Micro Kit (Qiagen). The results are comparable to the corresponding results from conventional cytogenetic methods.

Cites

No record.

References

No record.

Cite

APA: Copy

SHAHROKHI, FAHIMEH, MOHADES ARDEBILI, S.M., FARDI AZAR, Z., KAMYAB, AHMAD REZA, SHAMSIAN, E., HAYAT NOUSAEID, M., KAEINI MOGHADAM, Z., & MAHDIAN, R.. (2009). USE OF REAL-TIME PCR METHOD ON THE AMNIOCYTE SAMPLES FOR PRENATAL DIAGNOSIS OF DOWN SYNDROME. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 12(3), 17-31. SID. https://sid.ir/paper/81128/en

Vancouver: Copy

SHAHROKHI FAHIMEH, MOHADES ARDEBILI S.M., FARDI AZAR Z., KAMYAB AHMAD REZA, SHAMSIAN E., HAYAT NOUSAEID M., KAEINI MOGHADAM Z., MAHDIAN R.. USE OF REAL-TIME PCR METHOD ON THE AMNIOCYTE SAMPLES FOR PRENATAL DIAGNOSIS OF DOWN SYNDROME. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2009;12(3):17-31. Available from: https://sid.ir/paper/81128/en

IEEE: Copy

FAHIMEH SHAHROKHI, S.M. MOHADES ARDEBILI, Z. FARDI AZAR, AHMAD REZA KAMYAB, E. SHAMSIAN, M. HAYAT NOUSAEID, Z. KAEINI MOGHADAM, and R. MAHDIAN, “USE OF REAL-TIME PCR METHOD ON THE AMNIOCYTE SAMPLES FOR PRENATAL DIAGNOSIS OF DOWN SYNDROME,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 12, no. 3, pp. 17–31, 2009, [Online]. Available: https://sid.ir/paper/81128/en

Related Journal Papers