A NOVEL SENSITIVE MULTIPLEX RT-PCR ELISA METHOD FOR DETECTION OF HIV-1/HCV CO-INFECTION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KHANLARI Z.; RAVANSHAD M.; RASOULI MANOUCHEHR; ZIAEIAN M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2008 | Volume:11 | Issue:1-2 Page(s): 57-63

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,251

Download:

Cites:

Information Journal Paper

Title

A NOVEL SENSITIVE MULTIPLEX RT-PCR ELISA METHOD FOR DETECTION OF HIV-1/HCV CO-INFECTION

Author(s)

KHANLARI Z. | RAVANSHAD M. | RASOULI MANOUCHEHR | ZIAEIAN M. | Issue Writer Certificate

Keywords

HIV 1Q3

HCVQ3

CO-INFECTIONQ3

MULTIPLEX RT-PCR ELISAQ3

Abstract

Objective: Despite sensitive antibody based blood donor screening, infection can be transmitted during window period. Therefore sensitive methods based on nucleic acid tests (NATs) have been considered. The aim of this project was to design a more sensitive method for detection of PCR products and diagnosis of HIV-1/HCV CO-INFECTION accurately.Materials and Methods: After designing specific primers and probes, the Multiplex RT-PCR method was optimized and the PCR products were labeled with Digoxigenin. The PCR product was denatured under alkaline condition and was hybridized with the specific probe that had a biotin at 5' end, and then was added to streptavidin coated wells. After washing an antibody against DIG, conjugated with alkaline phosphates enzyme was used, following second washing, the substrate (ABTS) solution was added to each reaction well. Development of green color shows the positive where as no color shows negative results. Results: 35 samples were tested with the developed method including 27 positive samples, 8 confirmed negative and 4 standard panels. False negative or positive reactions were not observed. Conclusion: This method had acceptable sensitivity and specificity for detecting HCV and HIV-1 infections during window period, also the method can be quantified which can be used for the flow-up and treatment of patients. In addition to the very high sensitivity of the test, it is cost effective and takes less time to performe

Cites

No record.

References

Cite

APA: Copy

KHANLARI, Z., RAVANSHAD, M., RASOULI, MANOUCHEHR, & ZIAEIAN, M.. (2008). A NOVEL SENSITIVE MULTIPLEX RT-PCR ELISA METHOD FOR DETECTION OF HIV-1/HCV CO-INFECTION. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 11(1-2), 57-63. SID. https://sid.ir/paper/81138/en

Vancouver: Copy

KHANLARI Z., RAVANSHAD M., RASOULI MANOUCHEHR, ZIAEIAN M.. A NOVEL SENSITIVE MULTIPLEX RT-PCR ELISA METHOD FOR DETECTION OF HIV-1/HCV CO-INFECTION. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2008;11(1-2):57-63. Available from: https://sid.ir/paper/81138/en

IEEE: Copy

Z. KHANLARI, M. RAVANSHAD, MANOUCHEHR RASOULI, and M. ZIAEIAN, “A NOVEL SENSITIVE MULTIPLEX RT-PCR ELISA METHOD FOR DETECTION OF HIV-1/HCV CO-INFECTION,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 11, no. 1-2, pp. 57–63, 2008, [Online]. Available: https://sid.ir/paper/81138/en

Related Journal Papers