THE EFFECT OF SIMULTANEOUS CO-CULTURE AND SEQUENTIAL MEDIA ON THE DEVELOPMENT OF VITRIFIED ONE-CELL MOUSE EMBRYO

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MOVAGHAR B.; MOVAHEDIAN M.; BAHARVAND H.; REZAZADEH M.

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Journal: Pathobiology Research Year:2002 | Volume:5 | Issue:1 Page(s): 115-122

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Title

THE EFFECT OF SIMULTANEOUS CO-CULTURE AND SEQUENTIAL MEDIA ON THE DEVELOPMENT OF VITRIFIED ONE-CELL MOUSE EMBRYO

Author(s)

MOVAGHAR B. | MOVAHEDIAN M. | BAHARVAND H. | REZAZADEH M. | Issue Writer Certificate

Keywords

VITRIFICATIONQ3

CO-CULTUREQ3

ONE CELL MOUSE EMBRYOSQ4

SEQUENTIAL MEDIAQ4

Abstract

Purpose: Wide spread efforts have been mode to facilitate and compare cryopreservation methods needed for the long-term conservation of embryos. Improving the embryo's culture system is another purpose of many researchers.Co-culture and SEQUENTIAL MEDIA systems can improve the developmental rate of vitrified and non-vitrified embryos separately. The aim of this research was to study, the effect of simultaneous use of vero cell CO-CULTURE and SEQUENTIAL MEDIA on the development of one-cell mouse embryos.Materials and Methods: One-cell mouse embryos were taken from NMRI mice with vaginal plaque, by the flushing method 26 hours after the intraperitoneal injection of hCG. Half of the embryos were vitrified by 40% ethylene ghycol solution (EFS 40%), then thawed and transferred to "R1-R2"(experiment group I) and "R1-R2 + Vero" (experiment group 2) media. The remaining embryos weretransfered to the same media as the non-vitrified control group. The data was recorded within 120 hours of the cultivation period and analyzed using the Chi square and Fisher tests.Results and Discussion: The results were as fallows: in vitrified embryos, the developmental rate in the "R1-R2 +Vero" group was higer than the "R1-R2" alone and the CO-CULTURE improved the developmental rate of the embryos significantly. But there was no significant difference between the developmental rate of the CO-CULTUREd and the non CO-CULTUREd embryos in the non vitrified group. Therefore, simultaneous use of CO-CULTURE system and SEQUENTIAL MEDIA has not any positive effect on the development of non vitrified embryos. But in vitrified embryos it improves the development of embryos.

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APA: Copy

MOVAGHAR, B., MOVAHEDIAN, M., BAHARVAND, H., & REZAZADEH, M.. (2002). THE EFFECT OF SIMULTANEOUS CO-CULTURE AND SEQUENTIAL MEDIA ON THE DEVELOPMENT OF VITRIFIED ONE-CELL MOUSE EMBRYO. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 5(1), 115-122. SID. https://sid.ir/paper/81193/en

Vancouver: Copy

MOVAGHAR B., MOVAHEDIAN M., BAHARVAND H., REZAZADEH M.. THE EFFECT OF SIMULTANEOUS CO-CULTURE AND SEQUENTIAL MEDIA ON THE DEVELOPMENT OF VITRIFIED ONE-CELL MOUSE EMBRYO. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2002;5(1):115-122. Available from: https://sid.ir/paper/81193/en

IEEE: Copy

B. MOVAGHAR, M. MOVAHEDIAN, H. BAHARVAND, and M. REZAZADEH, “THE EFFECT OF SIMULTANEOUS CO-CULTURE AND SEQUENTIAL MEDIA ON THE DEVELOPMENT OF VITRIFIED ONE-CELL MOUSE EMBRYO,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 5, no. 1, pp. 115–122, 2002, [Online]. Available: https://sid.ir/paper/81193/en

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