THE EFFECT OF ARTEMETHER-INDUCED APOPTOSIS IN PROMASTIGOTES OF LEISHMANIA MAJOR (MRHO/IR/75/ER) UNDER IN-VITRO CONDITIONS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

EBRAHIMISADR PARISA; GHAFFARIFAR FATEMEH; MOHAMMAD HASSAN ZUHAIR; BEHESHTI NASIBEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2012 | Volume:15 | Issue:2 Page(s): 2-10

Download Full-Text

Persian Verion

View:

880

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF ARTEMETHER-INDUCED APOPTOSIS IN PROMASTIGOTES OF LEISHMANIA MAJOR (MRHO/IR/75/ER) UNDER IN-VITRO CONDITIONS

Author(s)

EBRAHIMISADR PARISA | GHAFFARIFAR FATEMEH | MOHAMMAD HASSAN ZUHAIR | BEHESHTI NASIBEH | Issue Writer Certificate

Keywords

LEISHMANIA MAJORQ2

ARTEMETHERQ2

APOPTOSISQ2

FLOW CYTOMETRYQ2

Abstract

Objective: Leishmaniasis is one of the significant causes of morbidity and mortality in several countries. It is an important problem in endemic areas such as Iran. The goal in treatment of leishmaniasis is to reduce the disease period and leave no evidence of any remaining scars or lesions. A derivative of artemisinin is ARTEMETHER. Scientists believe that the strong action of ARTEMETHER against parasites is due to the presence of an endoperoxide bridge. Due to problems in the treatment of LEISHMANIA MAJOR, in this research we have studied the effect of ARTEMETHER on LEISHMANIA MAJOR under in vitro conditions.Methods: Parasites were cultured in NNN and RPMI, after which ARTEMETHER at concentrations of 5, 10, 25, 50 and 100 mg/ml were used for the promastigote assay. APOPTOSIS was detected by FLOW CYTOMETRY and DNA ladder assay.Results: The inhibitory concentration (IC50) of ARTEMETHER was determined to be 25 mg/ml. The percentage of apoptotic promastigotes at 25 mg/ml of ARTEMETHER was 42.28. The results of DNA fragmentation show that exposure of LEISHMANIA MAJOR promastigote cells to 25 mg/ml of ARTEMETHER lead to DNA fragmentation.Conclusion: We have proven the effect of ARTEMETHER on APOPTOSIS of LEISHMANIA MAJOR by FLOW CYTOMETRY and the DNA ladder assay.

Cites

CYTOTOXIC EFFECT OF METHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ FRUIT ON THE LEISHMANIA MAJOR IN VITRO

References

No record.

Cite

APA: Copy

EBRAHIMISADR, PARISA, GHAFFARIFAR, FATEMEH, MOHAMMAD HASSAN, ZUHAIR, & BEHESHTI, NASIBEH. (2012). THE EFFECT OF ARTEMETHER-INDUCED APOPTOSIS IN PROMASTIGOTES OF LEISHMANIA MAJOR (MRHO/IR/75/ER) UNDER IN-VITRO CONDITIONS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 15(2), 2-10. SID. https://sid.ir/paper/81291/en

Vancouver: Copy

EBRAHIMISADR PARISA, GHAFFARIFAR FATEMEH, MOHAMMAD HASSAN ZUHAIR, BEHESHTI NASIBEH. THE EFFECT OF ARTEMETHER-INDUCED APOPTOSIS IN PROMASTIGOTES OF LEISHMANIA MAJOR (MRHO/IR/75/ER) UNDER IN-VITRO CONDITIONS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2012;15(2):2-10. Available from: https://sid.ir/paper/81291/en

IEEE: Copy

PARISA EBRAHIMISADR, FATEMEH GHAFFARIFAR, ZUHAIR MOHAMMAD HASSAN, and NASIBEH BEHESHTI, “THE EFFECT OF ARTEMETHER-INDUCED APOPTOSIS IN PROMASTIGOTES OF LEISHMANIA MAJOR (MRHO/IR/75/ER) UNDER IN-VITRO CONDITIONS,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 15, no. 2, pp. 2–10, 2012, [Online]. Available: https://sid.ir/paper/81291/en

Related Journal Papers