CYTOTOXIC EFFECT OF METHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ FRUIT ON THE LEISHMANIA MAJOR IN VITRO

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NOORMOHAMMADI H.; MAROUFI Y.; DABIRZADEH M.; MIRI A.H.; GERAILI A.

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Journal: Journal of Medicinal Plants Year:2016 | Volume:15 | Issue:58 Page(s): 182-187

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Title

CYTOTOXIC EFFECT OF METHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ FRUIT ON THE LEISHMANIA MAJOR IN VITRO

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Keywords

TERMINALIA CHEBULA RETZQ2

AMASTIGOTESQ1

LEISHMANIA MAJORQ1

PROMASTIGOTESQ1

Abstract

Background: Cutaneous leishmaniasis is one of major public health problem in many countries. Pentavalent antimonite components as first line treatment of cutaneous leishmaniasis has many limitations and side effects.Objective: The aim of this study was to investigatethe cytotoxic effect of methanolic extract of TERMINALIA CHEBULA RETZ fruit on the LEISHMANIA MAJOR in vitro.Methods: TERMINALIA CHEBULA RETZ fruit extract with 80, 125, 250 and 500 mg/ml concentrations with leishmania PROMASTIGOTES were added to 96-well plate as 4-time repeated and incubated in 21oC. Viability percent of PROMASTIGOTES was estimated by direct counting and MTT assay; after 24, 48 and 72 hours. TERMINALIA CHEBULA RETZ extract with80 and 250 mg/ml were added to infected macrophages. The mean of AMASTIGOTES in each macrophage was obtained after 24 and 48 hours. Statistical analysis of data was done by SPSS software and one-way ANOVA test.Results: viability percent of PROMASTIGOTES in groups treated by maximum and minimum concentrations (500 and 80 mg/ml) of TERMINALIA CHEBULA RETZ after 24 hours was 19.01% and 98.65%, respectively.Viability of this groups after 48hours was 7.81% and 97.26% and after 72 hours it was 31.61% and 97.26%, respectively. IC50 for 24 hours was 114.10 mg/ml. Cytotoxicity of TERMINALIA CHEBULA RETZ extract (80 and 250 mg/ml) in AMASTIGOTES after 24 hours was 40.35% and 29.99%; and after 48 hours was 45.21% and 41.11%, respectively.Conclusion: extract of TERMINALIA CHEBULA RETZ fruit has cytotoxic effect on the PROMASTIGOTES and AMASTIGOTES of L. major in vitro.

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APA: Copy

NOORMOHAMMADI, H., MAROUFI, Y., DABIRZADEH, M., MIRI, A.H., & GERAILI, A.. (2016). CYTOTOXIC EFFECT OF METHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ FRUIT ON THE LEISHMANIA MAJOR IN VITRO. JOURNAL OF MEDICINAL PLANTS, 15(58), 182-187. SID. https://sid.ir/paper/15500/en

Vancouver: Copy

NOORMOHAMMADI H., MAROUFI Y., DABIRZADEH M., MIRI A.H., GERAILI A.. CYTOTOXIC EFFECT OF METHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ FRUIT ON THE LEISHMANIA MAJOR IN VITRO. JOURNAL OF MEDICINAL PLANTS[Internet]. 2016;15(58):182-187. Available from: https://sid.ir/paper/15500/en

IEEE: Copy

H. NOORMOHAMMADI, Y. MAROUFI, M. DABIRZADEH, A.H. MIRI, and A. GERAILI, “CYTOTOXIC EFFECT OF METHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ FRUIT ON THE LEISHMANIA MAJOR IN VITRO,” JOURNAL OF MEDICINAL PLANTS, vol. 15, no. 58, pp. 182–187, 2016, [Online]. Available: https://sid.ir/paper/15500/en

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