IMMOBILIZATION OF ORGANOPHOSPHORUS HYDROLASE ON THE SURFACE OF BACILLUS SUBTILIS SPORES BY THE ADSORPTION METHOD

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KHANAMANI FALAHATI POUR SOUDEH; SAHEBGHADAM LOTFI ABBAS; AHMADIAN GHOLAMREZA; BAGHIZADEH AMIN

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Journal: Pathobiology Research Year:2015 | Volume:18 | Issue:1 Page(s): 39-53

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Title

IMMOBILIZATION OF ORGANOPHOSPHORUS HYDROLASE ON THE SURFACE OF BACILLUS SUBTILIS SPORES BY THE ADSORPTION METHOD

Author(s)

KHANAMANI FALAHATI POUR SOUDEH | SAHEBGHADAM LOTFI ABBAS | AHMADIAN GHOLAMREZA | BAGHIZADEH AMIN | Issue Writer Certificate

Keywords

ORGANOPHOSPHORUS HYDROLASEQ2

BACILLUS SUBTILISQ3

IMMOBILIZATIONQ3

ADSORPTIONQ2

SPOREQ1

Abstract

Objective: Organophosphorus (OPs) compounds are widely used in many pesticides, insecticides and chemical nerve agents. These compounds are hazardous for humans and the environment. Organophosphate hydrolase (OPH) is a homodimeric protein initially isolated fromPseudomonas diminuta MG and Flavobacterium species. This enzyme is able to degrade a broad spectrum of toxic OPs compounds. Using immobilized OPH commonly presents a variety of advantages versus the free form of the enzyme.Advantages include an increase in stability, cost reduction by simple recovery and reutilization of the enzyme, quick and easy separation of the reactant and product in the reaction medium.Methods: Plasmid pET-26b (+) was used to generate the OPH protein under the control of the T7lac promoter.E. coli BL21 (DE3) pLysS was used as the host for expression of the OPH enzyme. Recombinant OPH was secreted into the extracellular medium and the purified enzyme was immobilized on the surface ofBacillus subtilis SPOREs by the ADSORPTION method, for the first time.Results: Approximately 42% to 45% enzymatic activity was determined to be associated with SPOREs. Optimal pH and temperature of the enzyme were not altered by the presence of the SPOREs. Thermo and pH stabilities of the immobilized enzyme was higher than the free form of the enzyme.Conclusion: BACILLUS SUBTILIS SPOREs are safe for humans and the environment. Therefore this system can be considered an environmentally friendly biocatalyst for degradation of OPs.

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APA: Copy

KHANAMANI FALAHATI POUR, SOUDEH, SAHEBGHADAM LOTFI, ABBAS, AHMADIAN, GHOLAMREZA, & BAGHIZADEH, AMIN. (2015). IMMOBILIZATION OF ORGANOPHOSPHORUS HYDROLASE ON THE SURFACE OF BACILLUS SUBTILIS SPORES BY THE ADSORPTION METHOD. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 18(1), 39-53. SID. https://sid.ir/paper/81298/en

Vancouver: Copy

KHANAMANI FALAHATI POUR SOUDEH, SAHEBGHADAM LOTFI ABBAS, AHMADIAN GHOLAMREZA, BAGHIZADEH AMIN. IMMOBILIZATION OF ORGANOPHOSPHORUS HYDROLASE ON THE SURFACE OF BACILLUS SUBTILIS SPORES BY THE ADSORPTION METHOD. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2015;18(1):39-53. Available from: https://sid.ir/paper/81298/en

IEEE: Copy

SOUDEH KHANAMANI FALAHATI POUR, ABBAS SAHEBGHADAM LOTFI, GHOLAMREZA AHMADIAN, and AMIN BAGHIZADEH, “IMMOBILIZATION OF ORGANOPHOSPHORUS HYDROLASE ON THE SURFACE OF BACILLUS SUBTILIS SPORES BY THE ADSORPTION METHOD,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 18, no. 1, pp. 39–53, 2015, [Online]. Available: https://sid.ir/paper/81298/en

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