THE EFFECTS OF P-BENZOQUINONE AND HYDROQUINONE ON THE RUNX2 GENE EXPRESSION AND OSTEOBLASTIC DIFFERENTIATION OF HUMAN MARROW DERIVED MESENCHYMAL STEM CELLS

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ZOLGHADR FATEMEH; SADEGHIZADEH MAJID; AMIRIZADEH NASER; BEHMANESH MEHRDAD; HOSSEINKHANI SAMAN; AMANI MARYAM

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Journal: Pathobiology Research Year:2011 | Volume:14 | Issue:2 Page(s): 25-35

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Title

THE EFFECTS OF P-BENZOQUINONE AND HYDROQUINONE ON THE RUNX2 GENE EXPRESSION AND OSTEOBLASTIC DIFFERENTIATION OF HUMAN MARROW DERIVED MESENCHYMAL STEM CELLS

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Keywords

HUMAN MARROW DERIVED MESENCHYMAL STEM CELLS (MSC)Q2

RUNX2 GENEQ2

OSTEOGENIC DIFFERENTIATIONQ2

ρ-BENZOQUINONE (BQ)Q2

HYDROQUINONE (HQ)Q2

Abstract

Objective: Evaluating the effects of p-benzoquinone and hydroquinone on the RUNX2 expression and osteoblastic differentiation of human marrow derived mesenchymal stem cells (MSCs).Materials and Methods: Bone marrow MSCs obtained by cultivating marrow mononuclear cells, were exposed to 10 mM of either p-benzoquinone or hydroquinone. Following chemical treatment, RUNX2 GENE expression was assessed by Real-time RT PCR 1, 6, 24 and 48 hours later and OSTEOGENIC DIFFERENTIATION was analyzed using alizarin red and alkaline phosphatase staining methods on days 7 and 14 after ostegenic induction.Results: RUNX2 expression was significantly elevated (up to approximately 8 times) due to chemical exposure but the applied chemicals exert no considerable effect on MSCs OSTEOGENIC DIFFERENTIATION.Conclusion: According to the literature, despite the necessity of RUNX2 overexpression on the induction of OSTEOGENIC DIFFERENTIATION, but it is not sufficient for osteogenesis to occure so increase in RUNX2 expression observed in our study is not the indicator of the induced OSTEOGENIC DIFFERENTIATION. Instead, this elevated expression could be the sign of increased activity of the canonical Wnt signaling pathway thereby its involvement in the development of AML due to exposure to benzene and its metabolites. Moreover, this augmented expression of RUNX2 in MSCs can indicate the RUNX2 overexpression in myeloid progenitors as an expected similar effect of exposure to benzene and its metabolites to contribute in myeloid malignancies developed due to benzene exposure.

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APA: Copy

ZOLGHADR, FATEMEH, SADEGHIZADEH, MAJID, AMIRIZADEH, NASER, BEHMANESH, MEHRDAD, HOSSEINKHANI, SAMAN, & AMANI, MARYAM. (2011). THE EFFECTS OF P-BENZOQUINONE AND HYDROQUINONE ON THE RUNX2 GENE EXPRESSION AND OSTEOBLASTIC DIFFERENTIATION OF HUMAN MARROW DERIVED MESENCHYMAL STEM CELLS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 14(2), 25-35. SID. https://sid.ir/paper/81316/en

Vancouver: Copy

ZOLGHADR FATEMEH, SADEGHIZADEH MAJID, AMIRIZADEH NASER, BEHMANESH MEHRDAD, HOSSEINKHANI SAMAN, AMANI MARYAM. THE EFFECTS OF P-BENZOQUINONE AND HYDROQUINONE ON THE RUNX2 GENE EXPRESSION AND OSTEOBLASTIC DIFFERENTIATION OF HUMAN MARROW DERIVED MESENCHYMAL STEM CELLS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2011;14(2):25-35. Available from: https://sid.ir/paper/81316/en

IEEE: Copy

FATEMEH ZOLGHADR, MAJID SADEGHIZADEH, NASER AMIRIZADEH, MEHRDAD BEHMANESH, SAMAN HOSSEINKHANI, and MARYAM AMANI, “THE EFFECTS OF P-BENZOQUINONE AND HYDROQUINONE ON THE RUNX2 GENE EXPRESSION AND OSTEOBLASTIC DIFFERENTIATION OF HUMAN MARROW DERIVED MESENCHYMAL STEM CELLS,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 14, no. 2, pp. 25–35, 2011, [Online]. Available: https://sid.ir/paper/81316/en

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