EVALUATION OF DIG-ELISA FOR DIAGNOSIS OF HUMAN HYDATIDOSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHAFFARIFAR FATEMEH; DEYLAMIASL A.A.H.; JALOSIAN F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2002 | Volume:4 | Issue:2 Page(s): 145-156

Download Full-Text

Persian Verion

View:

1,980

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF DIG-ELISA FOR DIAGNOSIS OF HUMAN HYDATIDOSIS

Author(s)

GHAFFARIFAR FATEMEH | DEYLAMIASL A.A.H. | JALOSIAN F. | Issue Writer Certificate

Keywords

HYDATIDOSISQ4

SERODIAGNOSISQ4

DIG-ELISAQ4

Abstract

Serologic methods are the most important techniques for the diagnosis of HYDATIDOSIS. The aim of this study was to evaluate the diffusion in Gel-ELISA (DIG-ELISA) for serodignosis of human HYDATIDOSIS. The DIG-ELlS A is a simple and sensitive ELISA test without the need for absorbance reader. The method combines the diffusion of antibodies in agar with the EIA concept in petri dishes. To standardize the test, 105 sera were evaluated in three groups: HYDATIDOSIS patients (40), healthy donors (20), and patients without other parasitic and non-parasitic diseases (45). DIGELISA was indicated as the most sensitive, specific and efficient method in 1:200 and 1:400 serum dilution. Hence diameter of reaction zones at those titers were selected for the cut-off (7:52 mm and 5.06 mm respectively). Sensitivity, specificity and efficiency of DIG-ELISA for 1:200 serum dilution was evaluated to be 95.2%, 95.6% and 95.4% and for 1:400 serum dilution 97.6%, 97% and 97.3% respectively. DIG-ELISA was found to be easy to setup in a moderately equipped laboratory. This test proved to be useful for field diagnosis considering the advantage of eliminating the need of an absorbance reader.

Cites

No record.

References

No record.

Cite

APA: Copy

GHAFFARIFAR, FATEMEH, DEYLAMIASL, A.A.H., & JALOSIAN, F.. (2002). EVALUATION OF DIG-ELISA FOR DIAGNOSIS OF HUMAN HYDATIDOSIS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 4(2), 145-156. SID. https://sid.ir/paper/81338/en

Vancouver: Copy

GHAFFARIFAR FATEMEH, DEYLAMIASL A.A.H., JALOSIAN F.. EVALUATION OF DIG-ELISA FOR DIAGNOSIS OF HUMAN HYDATIDOSIS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2002;4(2):145-156. Available from: https://sid.ir/paper/81338/en

IEEE: Copy

FATEMEH GHAFFARIFAR, A.A.H. DEYLAMIASL, and F. JALOSIAN, “EVALUATION OF DIG-ELISA FOR DIAGNOSIS OF HUMAN HYDATIDOSIS,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 4, no. 2, pp. 145–156, 2002, [Online]. Available: https://sid.ir/paper/81338/en

Related Journal Papers