EFFECTS OF INSULIN AND ASCORBIC ACID ON INHIBITION OF THE APOPTOSIS IN HIPPOCAMPUS OF STZ-INDUCED DIABETIC RATS

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JAFARI ANAR KOULI I.; SANKIAN M.; VARASTEH A.A.R.; HAGHIR H.

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Journal: Anatomical Sciences Journal Year:2010 | Volume:7 | Issue:28-29 Page(s): 133-143

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Title

EFFECTS OF INSULIN AND ASCORBIC ACID ON INHIBITION OF THE APOPTOSIS IN HIPPOCAMPUS OF STZ-INDUCED DIABETIC RATS

Author(s)

JAFARI ANAR KOULI I. | SANKIAN M. | VARASTEH A.A.R. | HAGHIR H. | Issue Writer Certificate

Keywords

DIABETESQ2

APOPTOSISQ3

ASCORBIC ACIDQ3

INSULINQ2

HIPPOCAMPUSQ3

Abstract

Purpose: The aim of this study was to investigate effects of INSULIN and ASCORBIC ACID on rate of Caspase – 3 activity and DNA Laddering in HIPPOCAMPUS of STZ-induced diabetic rats.Materials and Methods: Thirty male Wistar rats in five groups, 6 in each group: one control group (group C) and four diabetic groups [diabetic control (group D), treatment with INSULIN (group I), with ASCORBIC ACID (group AA) and with INSULIN plus ASCORBIC ACID (group I+AA)] were used in this study. DIABETES was induced by injection of 60 mg/kg STZ IP. After six weeks, rats in group I were treated with INSULIN (4-6 U/kg/day Sc.), rats in group AA treated with ASCORBIC ACID (200 mg/kg/day, IP) and rats in group I+AA treated with equal dosage of both INSULIN and ASCORBIC ACID for two weeks. Rats in group D were treated with saline and considered as the diabetic control group. Two weeks after treatment, animals were anesthetized and HIPPOCAMPUS was dissected from hemispheres. Caspase-3 activity was assessed by Fluorometry, and finally, DNA fragmentation due to APOPTOSIS was determined by DNA laddering Assay.Results: Caspase-3 activity in group D significantly increased compared to group C (6.7 fold), whereas it decreased after treatment with INSULIN, ASCORBIC ACID or both (2.6, 4.2 and 5.1 fold, respectively). DNA laddering was observed in group D, but not in three treated groups.Conclusions: From this survey it was concluded that treatment of STZ-induced diabetic rats with INSULIN and/or L-ascorbic acid could possibly inhibit APOPTOSIS in hippocampal tissues using decrease of Caspase -3 activity and prevention of DNA Laddering.

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APA: Copy

JAFARI ANAR KOULI, I., SANKIAN, M., VARASTEH, A.A.R., & HAGHIR, H.. (2010). EFFECTS OF INSULIN AND ASCORBIC ACID ON INHIBITION OF THE APOPTOSIS IN HIPPOCAMPUS OF STZ-INDUCED DIABETIC RATS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 7(28-29), 133-143. SID. https://sid.ir/paper/81385/en

Vancouver: Copy

JAFARI ANAR KOULI I., SANKIAN M., VARASTEH A.A.R., HAGHIR H.. EFFECTS OF INSULIN AND ASCORBIC ACID ON INHIBITION OF THE APOPTOSIS IN HIPPOCAMPUS OF STZ-INDUCED DIABETIC RATS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2010;7(28-29):133-143. Available from: https://sid.ir/paper/81385/en

IEEE: Copy

I. JAFARI ANAR KOULI, M. SANKIAN, A.A.R. VARASTEH, and H. HAGHIR, “EFFECTS OF INSULIN AND ASCORBIC ACID ON INHIBITION OF THE APOPTOSIS IN HIPPOCAMPUS OF STZ-INDUCED DIABETIC RATS,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 7, no. 28-29, pp. 133–143, 2010, [Online]. Available: https://sid.ir/paper/81385/en

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