MOLECULAR CLONING AND MUTAGENESIS OF RAT GLUCOCEREBROSIDASE GENE

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NADERIAN H.; KAZEMI BAHRAM; HOSSEINI AHMAD; SADEGHI Y.; DE VRIES A.F.

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Journal: Anatomical Sciences Journal Year:2005 | Volume:3 | Issue:3 Page(s): 185-190

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Information Journal Paper

Title

MOLECULAR CLONING AND MUTAGENESIS OF RAT GLUCOCEREBROSIDASE GENE

Author(s)

Keywords

GLUCOCEREBROSIDASEQ4

CLONINGQ3

PUCBM21 IN FRAME MUTATIONQ4

Abstract

Purpose: The aim of this study was CLONING the Gba enzyme in pUCBM21 plasmid, and making frame mutation on it and sequencing it.Materials and methods: mRNA was extracted from mouse spleen and GLUCOCEREBROSIDASE cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restriction enzyme .Then frame mutation was prepared and sequenced.Results: GLUCOCEREBROSIDASE cDNA was synthesized and amplified by PCR. Then cloned in pUCBM21 and confirmed using restriction analysis. An in frame mutation was done and confirmed by sequencing.Conclusion: The successful sequenced Gba cDNA compared with other researchers results.

Cites

SUB CLONING OF MOUSE MOUSCULUS GLUCOCEREBROSIDASE ENZYME GENE IN LENTIVIRAL VECTOR AND TRANSFER TO HEK CELL LINE

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APA: Copy

NADERIAN, H., KAZEMI, BAHRAM, HOSSEINI, AHMAD, SADEGHI, Y., & DE VRIES, A.F.. (2005). MOLECULAR CLONING AND MUTAGENESIS OF RAT GLUCOCEREBROSIDASE GENE. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 3(3), 185-190. SID. https://sid.ir/paper/81439/en

Vancouver: Copy

NADERIAN H., KAZEMI BAHRAM, HOSSEINI AHMAD, SADEGHI Y., DE VRIES A.F.. MOLECULAR CLONING AND MUTAGENESIS OF RAT GLUCOCEREBROSIDASE GENE. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2005;3(3):185-190. Available from: https://sid.ir/paper/81439/en

IEEE: Copy

H. NADERIAN, BAHRAM KAZEMI, AHMAD HOSSEINI, Y. SADEGHI, and A.F. DE VRIES, “MOLECULAR CLONING AND MUTAGENESIS OF RAT GLUCOCEREBROSIDASE GENE,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 3, no. 3, pp. 185–190, 2005, [Online]. Available: https://sid.ir/paper/81439/en

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