MOLECULAR DETECTION OF 16S RRNA, QACA / B, SMR IN CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE

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Tahmasebi Hamed; BOKAEIAN MOHAMMAD

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Journal: Journal of Sabzevar University of Medical Sciences Year:2018 | Volume:24 | Issue:5 Page(s): 367-374

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Information Journal Paper

Title

MOLECULAR DETECTION OF 16S RRNA, QACA / B, SMR IN CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE

Author(s)

Tahmasebi Hamed | BOKAEIAN MOHAMMAD | Issue Writer Certificate

Keywords

QACA-BQ2

SMRQ1

MECAQ1

STAPHYLOCOCCUS AUREUSQ1

Abstract

Objectives: Disinfectants and antiseptics agents of the past were used for disinfect equipment and surfaces. In some cases, these substances ineffective by a series of specific efflux proteins that are present in bacteria. QqcA / B and SMR such as most important genes those can be expression more efflux protein in strain of methicillin-resistant STAPHYLOCOCCUS AUREUS (MRSA). The aim of this study was to investigate the genes qacA / B and SMR is MRSA strains.Methods: 89 isolates from various clinical samples were early identified by biochemical test. Initial screening was performed by Cefoxitin disk diffusion (30 mg) and then detection of MECA, SMR and qacA/B gens was done by polymerase chain reaction (PCR). Finally, were analyzed results by using chi-square tests and t-test.Results: Out of 89 STAPHYLOCOCCUS AUREUS isolates, 59 isolates were identified methicillin-resistant by initial phenotype test. Among those, 50 isolates (17/56%) have respectively MECA gene, 14 isolates (73.15%) SMR gene and 29 isolates (58/32%) qacA / B gene. Using by statistical tests and p. value test, was obtained the relationship between qacA / B and MECA genes.Conclusions: According to the results of the studie of prevalence of genes in Zahedan and high frequency of MRSA strains, should pay more attention to the use of disinfectants agents.

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APA: Copy

Tahmasebi, Hamed, & BOKAEIAN, MOHAMMAD. (2018). MOLECULAR DETECTION OF 16S RRNA, QACA / B, SMR IN CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 24(5), 367-374. SID. https://sid.ir/paper/82331/en

Vancouver: Copy

Tahmasebi Hamed, BOKAEIAN MOHAMMAD. MOLECULAR DETECTION OF 16S RRNA, QACA / B, SMR IN CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;24(5):367-374. Available from: https://sid.ir/paper/82331/en

IEEE: Copy

Hamed Tahmasebi, and MOHAMMAD BOKAEIAN, “MOLECULAR DETECTION OF 16S RRNA, QACA / B, SMR IN CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 5, pp. 367–374, 2018, [Online]. Available: https://sid.ir/paper/82331/en

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