TELOMERASE ACTIVITY IN BREAST CANCER CELLS UNDER TREATMENT WITH CROCIN

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KHOSROJERDI F.; KAZEMI NOUREINI S.; NABIUNI M.; NOURI Z.

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Paper Information

Journal: Journal of Sabzevar University of Medical Sciences Year:2012 | Volume:19 | Issue:3 (65) Page(s): 267-276

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Information Journal Paper

Title

TELOMERASE ACTIVITY IN BREAST CANCER CELLS UNDER TREATMENT WITH CROCIN

Author(s)

KHOSROJERDI F. | KAZEMI NOUREINI S. | NABIUNI M. | NOURI Z. | Issue Writer Certificate

Keywords

TELOMERASEQ2

CROCINQ1

BREAST NEOPLASMSQ1

ADENOCARCINOMAQ3

Abstract

Background: CROCIN is a major constituent of saffron, the dried stigmas of Crocus sativus L., which is used mainly as a herbal medicine or a food coloring agent around the world. Novel publications reporting a cancer preventive effect for CROCIN motivated us to evaluate TELOMERASE activity, the main cause of immortality in cancer cells, under treatment with CROCIN.Materials and Methods: IC50 concentration of CROCIN was estimated in MCF-7 cell line, a breast ADENOCARCINOMA cancer model, by MTT (3- (4, 5-dimethylthiazol-2-yl) -2, 5- diphenyltetrazolium bromide) test after 48 hours of treatment. A conventional telomere repeat amplification protocol (TRAP) assay and a real-time quantitative telomere repeat amplification protocol (qTRAP) assay were used to estimate relative TELOMERASE activity in CROCIN-treated cells in comparison with untreated control cells.Results: TELOMERASE activity in the treated cells with different concentrations of CROCIN up to IC50 showed an increment after administration of very low doses of CROCIN, whereas higher concentrations of CROCIN remarkably inhibited the enzyme in a dose-dependent manner. IC50 values of CROCIN reduced by 85% in comparison with untreated control cells.Conclusion: qTRAP estimations show a good correlation with the conventional assay results. Antiproliferative effect of CROCIN in cancer cells is probably due to strong inhibition of TELOMERASE activity.

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APA: Copy

KHOSROJERDI, F., KAZEMI NOUREINI, S., NABIUNI, M., & NOURI, Z.. (2012). TELOMERASE ACTIVITY IN BREAST CANCER CELLS UNDER TREATMENT WITH CROCIN. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 19(3 (65)), 267-276. SID. https://sid.ir/paper/82632/en

Vancouver: Copy

KHOSROJERDI F., KAZEMI NOUREINI S., NABIUNI M., NOURI Z.. TELOMERASE ACTIVITY IN BREAST CANCER CELLS UNDER TREATMENT WITH CROCIN. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2012;19(3 (65)):267-276. Available from: https://sid.ir/paper/82632/en

IEEE: Copy

F. KHOSROJERDI, S. KAZEMI NOUREINI, M. NABIUNI, and Z. NOURI, “TELOMERASE ACTIVITY IN BREAST CANCER CELLS UNDER TREATMENT WITH CROCIN,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 19, no. 3 (65), pp. 267–276, 2012, [Online]. Available: https://sid.ir/paper/82632/en

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