FREQUENCIES OF SUL1, SUL2, SUL3, DRF7 GENES IN CO-TRIMOXAZOLE RESISTANCE IN GRAM-NEGATIVE BACILLI ISOLATED FROM CLINICAL SPECIMENS OF HOSPITALIZED PATIENTS IN PARS GENERAL HOSPITAL, TEHRAN, IRAN

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MOHAMMADI F.; NOORBAKHSH F.; HONARMAND JAHROMI S.

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Journal: JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH Year:2017 | Volume:15 | Issue:3 Page(s): 291-302

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Title

FREQUENCIES OF SUL1, SUL2, SUL3, DRF7 GENES IN CO-TRIMOXAZOLE RESISTANCE IN GRAM-NEGATIVE BACILLI ISOLATED FROM CLINICAL SPECIMENS OF HOSPITALIZED PATIENTS IN PARS GENERAL HOSPITAL, TEHRAN, IRAN

Author(s)

MOHAMMADI F. | NOORBAKHSH F. | HONARMAND JAHROMI S. | Issue Writer Certificate

Keywords

COTRIMOXAZOLEQ1

GRAM-NEGATIVE BACILLIQ1

ANTIBIOTIC RESISTANCEQ2

SUL1Q1

SUL2Q1

SUL3Q1

DRF7 GENESQ1

Abstract

Background and Aim: In the last few decades co-trimoxazole, an antibacterial combination of trimethoprim and sulfamethoxazole, has been used for treatment of bacterial infections, but due to the vast usage of these drugs, resistant strains have appeared throughout the world.One of the reasons for resistance to co-trimoxazole is related todrf genes, which are responsible for trimethoprim resistance, while the sulfamethoxazole resistance is due to sulfonamidesul genes. The aim of this study was to investigate drug resistance and frequencies of resistance genes in gram-negative bacteria isolated from clinical specimens.Materials and Methods: Clinical samples were collected from patients in Pars Hospital, Tehran, Iran, in which presence of gram-negative bacteria was confirmed by biochemical tests. Then antibiotic susceptibility tests were performed for 5 antibiotics by disk diffusion agar technic. DNA was extracted from bacteria resistant to co-trimoxazole, followed by PCR using specific primers for SUL1, SUL2, SUL3, and DRF7 GENES.Results: In the co-trimoxazole-resistant bacteria, 26%, 74%, 2% and 16% of the isolates contained SUL1 gene, SUL2 gene, SUL3 gene and drf7 gene, respectively. Further analysis of the data showed that 51% of the isolates were resistant to gentamicin, 74% to ceftriaxone, 65% to ciprofloxacin and 3% to colistin. For resistance totrimethoprim only the drf 7 gene was used.Conclusion: The results of this study show that in the isolates of co-trimoxazole-resistant gram-negative bacteria, the sul 2 gene has a major role in development of resistance to sulfonamides. In this study onlythe drf 7 gene was used to assess the resistance of trimethoprim, sowe recommend to conduct studies also on other drf genes, so that the importance of each in resistance toco-trimoxazole can be determined.

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APA: Copy

MOHAMMADI, F., NOORBAKHSH, F., & HONARMAND JAHROMI, S.. (2017). FREQUENCIES OF SUL1, SUL2, SUL3, DRF7 GENES IN CO-TRIMOXAZOLE RESISTANCE IN GRAM-NEGATIVE BACILLI ISOLATED FROM CLINICAL SPECIMENS OF HOSPITALIZED PATIENTS IN PARS GENERAL HOSPITAL, TEHRAN, IRAN. JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH, 15(3 ), 291-302. SID. https://sid.ir/paper/85026/en

Vancouver: Copy

MOHAMMADI F., NOORBAKHSH F., HONARMAND JAHROMI S.. FREQUENCIES OF SUL1, SUL2, SUL3, DRF7 GENES IN CO-TRIMOXAZOLE RESISTANCE IN GRAM-NEGATIVE BACILLI ISOLATED FROM CLINICAL SPECIMENS OF HOSPITALIZED PATIENTS IN PARS GENERAL HOSPITAL, TEHRAN, IRAN. JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH[Internet]. 2017;15(3 ):291-302. Available from: https://sid.ir/paper/85026/en

IEEE: Copy

F. MOHAMMADI, F. NOORBAKHSH, and S. HONARMAND JAHROMI, “FREQUENCIES OF SUL1, SUL2, SUL3, DRF7 GENES IN CO-TRIMOXAZOLE RESISTANCE IN GRAM-NEGATIVE BACILLI ISOLATED FROM CLINICAL SPECIMENS OF HOSPITALIZED PATIENTS IN PARS GENERAL HOSPITAL, TEHRAN, IRAN,” JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH, vol. 15, no. 3 , pp. 291–302, 2017, [Online]. Available: https://sid.ir/paper/85026/en

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