DETECTION OF SUL2 GENE IN ESCHERICHIA COLI ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTIONS ADMITTED TO CLINICAL CENTERS OF KHOY CITY

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NOROUZI J.; AKHAVAN SEPAHY A.; BAZZAZZADEH N.

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Journal: Journal of Advances in Medical and Biomedical Research Year:2014 | Volume:21 | Issue:88 Page(s): 76-83

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Title

DETECTION OF SUL2 GENE IN ESCHERICHIA COLI ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTIONS ADMITTED TO CLINICAL CENTERS OF KHOY CITY

Author(s)

NOROUZI J. | AKHAVAN SEPAHY A. | BAZZAZZADEH N. | Issue Writer Certificate

Keywords

ESCHERICHIA COLIQ2

SULFONAMIDESQ2

SUL2 GENEQ2

ANTIBIOTIC RESISTANCEQ2

DISK-DIFFUSION METHODQ2

MICQ2

Abstract

Background and Objective: Widespread use of co-trimoxazole in urinary tract infection treatments and its incorrect use has led to the emergence of co-trimoxazole resistant E. coli strains. The aim of this study was to evaluate SUL2 GENE among E. coli isolates of patients admitted to the clinical centers of Khoy city.Materials and Methods: Three hundred urine samples were collected from clinical centers of Khoy city. Among them, 100 E.coli isolates were confirmed by standard biochemical tests. Furthermore, the antibiotic susceptibility tests to 10 antibiotics were performed by the-disk-agar diffusion (DAD) method. Also, MIC and MBC were evaluated for co-trimoxazole by MICrodilution broth method. Finally, PCR was done in order to find SUL2 GENE in resistant isolates.Results: Of 100 isolates, 71 (71%) were resistant to co-trimoxazole out of which 57 (80%) had SUL2 GENE. This gene was recognized in SULFONAMIDES-resistant isolates which did not create any zone of inhibition. Also, the isolates with SUL2 GENE were found to be simultaneously resistant to ampicillin, nalidixic acid and ciprofloxacin. In co-trimoxazole-resistant isolates, MIC and MBC for Co-trimoxazole were calculated 16 and 32mg/ml.Conclusion: This study was the first of its kind in Iran with the main objective of manifesting the presence of SUL2 GENE in E. coli. The results of the present study indicate that antibiotic-resistance percentage in Khoy city reaches to 71%. In such a situation, it is inevitable to see resistance to all common antibiotics currently prescribed to patients at clinical centers which will, in turn, result in treatment failures.

Cites

FREQUENCIES OF SUL1, SUL2, SUL3, DRF7 GENES IN CO-TRIMOXAZOLE RESISTANCE IN GRAM-NEGATIVE BACILLI ISOLATED FROM CLINICAL SPECIMENS OF HOSPITALIZED PATIENTS IN PARS GENERAL HOSPITAL, TEHRAN, IRAN

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APA: Copy

NOROUZI, J., AKHAVAN SEPAHY, A., & BAZZAZZADEH, N.. (2014). DETECTION OF SUL2 GENE IN ESCHERICHIA COLI ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTIONS ADMITTED TO CLINICAL CENTERS OF KHOY CITY. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, 21(88), 76-83. SID. https://sid.ir/paper/61620/en

Vancouver: Copy

NOROUZI J., AKHAVAN SEPAHY A., BAZZAZZADEH N.. DETECTION OF SUL2 GENE IN ESCHERICHIA COLI ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTIONS ADMITTED TO CLINICAL CENTERS OF KHOY CITY. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH[Internet]. 2014;21(88):76-83. Available from: https://sid.ir/paper/61620/en

IEEE: Copy

J. NOROUZI, A. AKHAVAN SEPAHY, and N. BAZZAZZADEH, “DETECTION OF SUL2 GENE IN ESCHERICHIA COLI ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTIONS ADMITTED TO CLINICAL CENTERS OF KHOY CITY,” JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, vol. 21, no. 88, pp. 76–83, 2014, [Online]. Available: https://sid.ir/paper/61620/en

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