EVALUATION OF CHEMICAL COMPOSITION OF HELICHRYSUM ARTEMISIOIDESEXTRACT ITS EFFECT ON BIOFILM FORMATION AND ICAD GENE EXPRESSION IN CLINICAL ISOLATES OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

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MIRZAIE A.; NOORBAZARGAN H.; KHATAMI H.; SADAT SHANDIZ A; RAHIMI A.; BAGHERI KASHTALI A.

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Journal: Journal of Ilam University of Medical Sciences Year:2017 | Volume:25 | Issue:1 Page(s): 180-194

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Title

EVALUATION OF CHEMICAL COMPOSITION OF HELICHRYSUM ARTEMISIOIDESEXTRACT ITS EFFECT ON BIOFILM FORMATION AND ICAD GENE EXPRESSION IN CLINICAL ISOLATES OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

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Keywords

HELICHRYSUM ARTEMISIODESQ1

STAPHYLOCOCCUS AREUSQ1

BIOFILMQ1

ICADQ1

Abstract

Introduction: Staphylococcus aureus is amajor causative agent of nosocomialinfections which produces a wide range of diseases due to BIOFILM formation. This study aimed to investigate the phytochemical composition of Helichrysum artemisioides extract, its anti-biofilm effects on clinicalisolates of methicillin-resistantStaphylococcus aureus, as well as, analysis oficaD gene expression.Materials & methods: First, thephytochemical composition of H. artemisioides extract was studied by GC/MS method. Then, the BIOFILM formation capacity of 50 clinical isolates of S. aureus wasevaluated by Congo red agar and polymerasechain reaction (PCR). Finally, after treatmentof strains with Sub MIC concentration of the extract within 24 hours, the expression levelof ICAD gene was evaluated by Real Time PCR.Findings: The phytochemical analysis of extract showed 52 compounds, including α-Pinene (12.5%) and Carvacrol (8.6%) as the major components. The evaluation of BIOFILM formation test shows that out of 50 strains of S. aureus, 10 strains (20%) were positive for BIOFILMs formation. Also, after treatment ofselected strains by SubMIC concentration ofthe extract, the gene expression quantitation of ICAD was down-regulated significantly(P<0.05).Discusion & conclusions: Due to thesignificant effects of H. artemisioides extracton BIOFILM formation, it seems that the extractcould be used as complementary therapy toinhibit S. aureus BIOFILM.

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APA: Copy

MIRZAIE, A., NOORBAZARGAN, H., KHATAMI, H., SADAT SHANDIZ, A, RAHIMI, A., & BAGHERI KASHTALI, A.. (2017). EVALUATION OF CHEMICAL COMPOSITION OF HELICHRYSUM ARTEMISIOIDESEXTRACT ITS EFFECT ON BIOFILM FORMATION AND ICAD GENE EXPRESSION IN CLINICAL ISOLATES OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 25(1), 180-194. SID. https://sid.ir/paper/90082/en

Vancouver: Copy

MIRZAIE A., NOORBAZARGAN H., KHATAMI H., SADAT SHANDIZ A, RAHIMI A., BAGHERI KASHTALI A.. EVALUATION OF CHEMICAL COMPOSITION OF HELICHRYSUM ARTEMISIOIDESEXTRACT ITS EFFECT ON BIOFILM FORMATION AND ICAD GENE EXPRESSION IN CLINICAL ISOLATES OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;25(1):180-194. Available from: https://sid.ir/paper/90082/en

IEEE: Copy

A. MIRZAIE, H. NOORBAZARGAN, H. KHATAMI, A SADAT SHANDIZ, A. RAHIMI, and A. BAGHERI KASHTALI, “EVALUATION OF CHEMICAL COMPOSITION OF HELICHRYSUM ARTEMISIOIDESEXTRACT ITS EFFECT ON BIOFILM FORMATION AND ICAD GENE EXPRESSION IN CLINICAL ISOLATES OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 1, pp. 180–194, 2017, [Online]. Available: https://sid.ir/paper/90082/en

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