Optimized Mouse BMDC Isolation and Culture under Endotoxin-Free Conditions

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Sadeghi Shermeh A.; HABIBZADEH S.; TAGHIKHANI A.; RAFATI S.; SEYED N.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Ilam University of Medical Sciences Year:2021 | Volume:29 | Issue:2 Page(s): 43-54

Download Full-Text

Persian Verion

View:

314

Download:

Cites:

Information Journal Paper

Title

Optimized Mouse BMDC Isolation and Culture under Endotoxin-Free Conditions

Author(s)

Keywords

Bone marrow

Dendritic cells

Cell culture optimization

Endotoxin

Abstract

Introduction: Dendritic cells are very important in basic studies and vaccine research, but isolation and culture of these cells face challenges due to their small number in tissues. Since there is no standard method, we addressed some of the factors affecting the efficiency of dendritic cell isolation and culture from BALB/c mouse Bone marrow. Materials & Methods: Bone marrow cells isolated from femur and tibia, were seeded in plates and treated with GM-CSF. On day 3, half of the media was transferred to a new plate with fresh media plus GM-CSF. On day 5, cells were induced with CpG oligonucleotide. Cell survival as well as maturation markers of CD11C, MHC-II and CD86 were investigated by flow cytometry on day 5 (immature cells) and day 7 (mature cells). Findings: It was found that the presence of Endotoxin in the culture of immature cells, significantly reduces the recovery of Dendritic cells (p value <0. 05). Less than one million cells seeded in non-treated 6-well plates and transfer of cells to a new plate on day 3 increased the efficiency of immature cell retrieval by preventing early maturation and premature death. Further treatment of immature cells with CpG on day 7 was used to induce the Th1 pathway. Significant difference (p value <0. 05) with control group showed that CpG induces maturation after treatment with GM-CSF (without IL-4). Discussion & Conclusions: Optimization of dendritic cell culture in terms of different culture conditions highly impacts the efficiency of immature cell formation and maturation.

Cites

No record.

References

MicroRNA modified tumor derived exosomes as novel tools for maturation of dendritic cells

Cite

APA: Copy

Sadeghi Shermeh, A., HABIBZADEH, S., TAGHIKHANI, A., RAFATI, S., & SEYED, N.. (2021). Optimized Mouse BMDC Isolation and Culture under Endotoxin-Free Conditions. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 29(2 ), 43-54. SID. https://sid.ir/paper/950381/en

Vancouver: Copy

Sadeghi Shermeh A., HABIBZADEH S., TAGHIKHANI A., RAFATI S., SEYED N.. Optimized Mouse BMDC Isolation and Culture under Endotoxin-Free Conditions. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2021;29(2 ):43-54. Available from: https://sid.ir/paper/950381/en

IEEE: Copy

A. Sadeghi Shermeh, S. HABIBZADEH, A. TAGHIKHANI, S. RAFATI, and N. SEYED, “Optimized Mouse BMDC Isolation and Culture under Endotoxin-Free Conditions,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 29, no. 2 , pp. 43–54, 2021, [Online]. Available: https://sid.ir/paper/950381/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops