STUDY OF THE EFFECTS OF PLASMA LIPOPROTEINS IN PURIFICATION OF ANTI-RH(D) THROUGH COLUMNAR CHROMATOGRAPHY METHOD

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VARESTEH A.R.; HASHEMI M.; SABERPOUR F.

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Journal: Journal of Birjand University of Medical Sciences Year:2003 | Volume:10 | Issue:3 Page(s): 5-9

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Title

STUDY OF THE EFFECTS OF PLASMA LIPOPROTEINS IN PURIFICATION OF ANTI-RH(D) THROUGH COLUMNAR CHROMATOGRAPHY METHOD

Author(s)

VARESTEH A.R. | HASHEMI M. | SABERPOUR F. | Issue Writer Certificate

Keywords

LIPOPROTEIN; ANTI-D; ION EXCHANGE CHROMATOGRAPHY.Q3

Abstract

Background and Aim: Immunoglobulins prepared by Ion Exchange Chromatography (IEC) from plasma are administered intravenously because of low anti-complementary activity and aggregation. Mostly separation of gamaglobulins in plasma is difficult because of lipoproteins. In IgG purification by IEC, plasma lipoproteins may change the capacity of IEC gel. In this research, the role of lipoproteins on the capacity of the IEC gel used for Anti-D IgG purification was studied. Materials and Methods: Two different methods were used to eliminate plasma lipoproteins and then, Anti-D was purified by IEC from normal and free lipoprotein plasma. Anti-D titer and ratio of IgG to total protein in purified Anti-D was also evaluated. Results: The results indicated that lipoproteins were eliminated considerably by Dextran-Silicagel method. Elimination of plasma lipoproteins increase the ratio of IgG to total protein in IEC purified Anti-D, whereas Anti-D titer was constant. Conclusion: Elimination of plasma lipoproteins increases the capacity of the IEC gel for purification of IgG from plasma. This is an economical advantage in industrial level purification and production of IgG due to decrease the final prices.

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APA: Copy

VARESTEH, A.R., HASHEMI, M., & SABERPOUR, F.. (2003). STUDY OF THE EFFECTS OF PLASMA LIPOPROTEINS IN PURIFICATION OF ANTI-RH(D) THROUGH COLUMNAR CHROMATOGRAPHY METHOD. JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES, 10(3), 5-9. SID. https://sid.ir/paper/95329/en

Vancouver: Copy

VARESTEH A.R., HASHEMI M., SABERPOUR F.. STUDY OF THE EFFECTS OF PLASMA LIPOPROTEINS IN PURIFICATION OF ANTI-RH(D) THROUGH COLUMNAR CHROMATOGRAPHY METHOD. JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2003;10(3):5-9. Available from: https://sid.ir/paper/95329/en

IEEE: Copy

A.R. VARESTEH, M. HASHEMI, and F. SABERPOUR, “STUDY OF THE EFFECTS OF PLASMA LIPOPROTEINS IN PURIFICATION OF ANTI-RH(D) THROUGH COLUMNAR CHROMATOGRAPHY METHOD,” JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES, vol. 10, no. 3, pp. 5–9, 2003, [Online]. Available: https://sid.ir/paper/95329/en

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