The Immunohistochemical Staining in Determination of Postoperative Bowel Dysfunction in Children with Hirschsprung's Disease

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Hoseinpour Mehradad; Mostafavi Sayed Mahmoud; Beyraghdar Mojdeh

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2020 | Volume:38 | Issue:587 Page(s): 582-588

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Title

The Immunohistochemical Staining in Determination of Postoperative Bowel Dysfunction in Children with Hirschsprung's Disease

Author(s)

Hoseinpour Mehradad | Mostafavi Sayed Mahmoud | Beyraghdar Mojdeh | Issue Writer Certificate

Keywords

Hirschsprung's disease

Immunohistochemistry

Calretinin

Nitric oxide

Abstract

Background: The aim of this study was to evaluate the Calretinin and Nitric oxide (NO) immunohistochemical (IHC) staining in ganglionic segment of colon of children with Hirschsprung's disease (HD). Methods: In a cross-sectional study, 60 children with HD entered the study, and underwent surgery to pull out the bowel from the anus to take biopsy from every 5 cm. Hematoxylin and eosin (H&E) and IHC methods were evaluated, and the results were reported to be poorly positive or severely positive for Calretinin. All patients were followed up 1 and 6 months after the operation, and the results were compared between the two groups. Findings: From 60 children with HD, 14 patients (23. 3%) were symptomatic. In 14 symptomatic patients, all colorectal samples from ganglionic segments were negative for NO staining in comparison of placental NO staining as sham group. In the IHC examination, nuclear and cytoplasmic Calretinin staining revealed ganglion cells in 52 patients as strongly positive, and weakly positive in 8 patients. Six patients in strongly positive group (11. 5%) were symptomatic, while 8 patients whose Calretinin staining was reported as weakly stained, had symptoms (P = 0. 001). Overall accuracy of prediction of postoperative constipation for Calretinin staining was 96. 67%. Conclusion: According to the findings of our study, it seems that in children with HD who undergo surgery, proximal ganglion immunohistochemical staining may be effective in determining postoperative bowel dysfunction.

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APA: Copy

Hoseinpour, Mehradad, Mostafavi, Sayed Mahmoud, & Beyraghdar, Mojdeh. (2020). The Immunohistochemical Staining in Determination of Postoperative Bowel Dysfunction in Children with Hirschsprung's Disease. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 38(587 ), 582-588. SID. https://sid.ir/paper/953584/en

Vancouver: Copy

Hoseinpour Mehradad, Mostafavi Sayed Mahmoud, Beyraghdar Mojdeh. The Immunohistochemical Staining in Determination of Postoperative Bowel Dysfunction in Children with Hirschsprung's Disease. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2020;38(587 ):582-588. Available from: https://sid.ir/paper/953584/en

IEEE: Copy

Mehradad Hoseinpour, Sayed Mahmoud Mostafavi, and Mojdeh Beyraghdar, “The Immunohistochemical Staining in Determination of Postoperative Bowel Dysfunction in Children with Hirschsprung's Disease,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 38, no. 587 , pp. 582–588, 2020, [Online]. Available: https://sid.ir/paper/953584/en

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