Evaluation of phenotypic and molecular analysis of biofilm production in streptococcus mutants producing dental plaque

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Gharekhani Fatemeh; Momen AmirHossein

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Journal Paper

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Journal: Journal of Neyshabur University of Medical Sciences Year:2019 | Volume:7 | Issue:3 (24) Page(s): 63-74

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Information Journal Paper

Title

Evaluation of phenotypic and molecular analysis of biofilm production in streptococcus mutants producing dental plaque

Author(s)

Gharekhani Fatemeh | Momen AmirHossein | Issue Writer Certificate

Keywords

streptococcus mutants

Biofilm

PCR

Tooth Decay

Abstract

Introduction Tooth Decay is one of the most important problems facing general health in different societies, the activity of acid-producing bacteria, especially streptococcus mutants, is the main cause of this complication. The creation of the Biofilms of the bacterium is subject to the presence of a specific enzyme called glucan sucrase or glucosyltransferase, coded by gtf gene. The aim of this study was to evaluate the genes of GtfB and GtfC of streptococcus mutants in the formation of dental plaque Biofilm. Materials and Methods Blood agar was used to isolate streptococcus mutants., . In this study, the Biofilm was formed on a polystyrene surface using a microtiter plate and stained with a crystal violet through an ELISA reader at a wavelength of 550 nm. In order to confirm streptococcus mutants colonies, biochemical and fermentative tests were performed., the samples were transferred to 1% agarose gel and examined after dye gel staining. Results The results showed that out of 46 samples, 19 isolates (41. 30%) had gtfB gene and 8 (17. 4%) had gtfC gene. In this study, the highest growth rate of streptococcus mutants isolated from tooth plaque was obtained at a concentration of 0. 5 g / L sucrose and pH = 4. 5. Conclusion Therefore, sucrose sugar, which is commonly found in the diet, causes the growth of streptococcus mutants, which is the main cause of dental caries.

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APA: Copy

Gharekhani, Fatemeh, & Momen, AmirHossein. (2019). Evaluation of phenotypic and molecular analysis of biofilm production in streptococcus mutants producing dental plaque. JOURNAL OF NEYSHABUR UNIVERSITY OF MEDICAL SCIENCES, 7(3 (24) ), 63-74. SID. https://sid.ir/paper/954436/en

Vancouver: Copy

Gharekhani Fatemeh, Momen AmirHossein. Evaluation of phenotypic and molecular analysis of biofilm production in streptococcus mutants producing dental plaque. JOURNAL OF NEYSHABUR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;7(3 (24) ):63-74. Available from: https://sid.ir/paper/954436/en

IEEE: Copy

Fatemeh Gharekhani, and AmirHossein Momen, “Evaluation of phenotypic and molecular analysis of biofilm production in streptococcus mutants producing dental plaque,” JOURNAL OF NEYSHABUR UNIVERSITY OF MEDICAL SCIENCES, vol. 7, no. 3 (24) , pp. 63–74, 2019, [Online]. Available: https://sid.ir/paper/954436/en

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