Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Ganjalishahi Fatemeh; Hemmatpour Anahid; DEHGHAN MANSHADI MAHDI; Hadi Nedoushan Hossein; Karimollah Alireza

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2021 | Volume:43 | Issue:3 Page(s): 246-253

Download Full-Text

Persian Verion

View:

321

Download:

Cites:

Information Journal Paper

Title

Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals

Author(s)

Keywords

Paroxetine

PBMC

TLR2

TLR4

Abstract

Background: Paroxetine is one of the well-known antidepressants. Recent studies have focused on paroxetine’ s probable immuno-modulatory effects, since findings have indicated inflammation’ s role in the pathophysiology of depression. Therefore, in the present study, TLR2 and TLR4 mRNA genes expression was assessed in paroxetinetreated peripheral blood mononuclear cells (PBMCs). Methods: Venous blood samples were drawn from five healthy men (20-40 years old). Peripheral blood mononuclear cells (PBMCs) were isolated from samples and were cultured. After the first incubation for 24h, phytohemagglutinin plus lipopolysaccharide were added to the cells and then were incubated for 24h. Thereafter, cells were treated with different concentrations of paroxetine in the presence or absence of inhibitors of 5-HT2 and 5-HT7 receptors. After incubation for 48h, RNA was extracted and cDNA was synthesized. Using the real-Time PCR technique, TLR2 and TLR4 genes mRNA expression were evaluated. Statistical analysis of data were carried out using GraphPad Prism 7. Results: TLR2 and TLR4 mRNA expression were significantly increased in response to paroxetine at all concentrations. Furthermore, the co-culture of cells with the drug and the 5-HT2R and 5HT7R inhibitor simultaneously revealed that paroxetine’ s immuno-modulatory effects via TLR2 are dependent on serotonin, while it is independent of serotonin in the case of TLR4. Conclusion: Considering paroxetine’ s effect in modulating immune responses via increasing TLR2 and TLR4 expression, paroxetine could have therapeutic potentials in diseases with a deficiency in these receptors.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

Ganjalishahi, Fatemeh, Hemmatpour, Anahid, DEHGHAN MANSHADI, MAHDI, Hadi Nedoushan, Hossein, & Karimollah, Alireza. (2021). Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 43(3 ), 246-253. SID. https://sid.ir/paper/958319/en

Vancouver: Copy

Ganjalishahi Fatemeh, Hemmatpour Anahid, DEHGHAN MANSHADI MAHDI, Hadi Nedoushan Hossein, Karimollah Alireza. Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2021;43(3 ):246-253. Available from: https://sid.ir/paper/958319/en

IEEE: Copy

Fatemeh Ganjalishahi, Anahid Hemmatpour, MAHDI DEHGHAN MANSHADI, Hossein Hadi Nedoushan, and Alireza Karimollah, “Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 43, no. 3 , pp. 246–253, 2021, [Online]. Available: https://sid.ir/paper/958319/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops