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Information Journal Paper

Title

Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells

Pages

  187-192

Abstract

 Statement of the Problem: The stimulation of odontogenic activity is considered an essen-tial property for biomaterials used in vital pulp therapy. Purpose: The present study aimed to evaluate the effect of the incorporation of Zeolite con-taining silver-zinc Nanoparticles (Ze-Ag-Zn) into Angelous mineral trioxide aggregate (AM-TA) on the odontogenic activity of human Dental pulp stem cells (HDPSCs). Materials and Method: In this in vitro study, HDPSCs were treated with 2% wt of synthe-sized Ze-Ag-Zn particles+AMTA, AMTA and Ze-Ag-Zn disks. The negative control cells did not receive any treatment. Then, cell viability was measured using the MTT assay after 7 and 14 days of the treatment course. The Alkaline phosphatase (ALP) activity and calcium ion level were also measured in the supernatant culture media using auto-analyzer kits. The obtained data were analyzed using one-way ANOVA and Student t-test where appropriate. The level of the statistical significance was set at p< 0. 05. Results: The results indicated that HDPSCs treated with AMTA and AMTA+Ze-Ag-Zn particles did not show any significant cell death compared with the control cells after 14 days of the treatment course while the ALP activity and calcium ion levels were significantly (p< 0. 05) elevated. Also, the addition of AMTA particles to the cell culture media resulted in increased ALP activity and calcium ion level compared with HDPSCs treated with AMTA+ Ze-Ag-Zn particles on day 7 of the treatment course (p< 0. 05). Conclusion: It seems that the incorporation of Ze-Ag-Zn particles into AMTA did not have any significant positive effect on the Biomineralization properties of AMTA.

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    Cite

    APA: Copy

    GHASEMI, NEGIN, Salarinasab, Sadegh, RAHBARGHAZI, REZA, Sedghi, Samin, & Davoudi, Paria. (2021). Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells. JOURNAL OF DENTISTRY (SHIRAZ UNIVERSITY OF MEDICAL SCIENCES), 22(3), 187-192. SID. https://sid.ir/paper/975356/en

    Vancouver: Copy

    GHASEMI NEGIN, Salarinasab Sadegh, RAHBARGHAZI REZA, Sedghi Samin, Davoudi Paria. Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells. JOURNAL OF DENTISTRY (SHIRAZ UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2021;22(3):187-192. Available from: https://sid.ir/paper/975356/en

    IEEE: Copy

    NEGIN GHASEMI, Sadegh Salarinasab, REZA RAHBARGHAZI, Samin Sedghi, and Paria Davoudi, “Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells,” JOURNAL OF DENTISTRY (SHIRAZ UNIVERSITY OF MEDICAL SCIENCES), vol. 22, no. 3, pp. 187–192, 2021, [Online]. Available: https://sid.ir/paper/975356/en

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