In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Hasna Amjad Abu; Khoury Rayana Duarte; Toia Cassia Cestari; Goncalves Glaucia Beatriz; de Andrade Flaviana Bombarda; Talge Carvalho Claudio Antonio; Ribeiro Camargo Carlos Henrique; Valera Marcia Carneiro

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Endodontic Journal Year:2020 | Volume:15 | Issue:4 Page(s): 236-245

Download Full-Text

View:

111

Download:

Cites:

Information Journal Paper

Title

In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis

Author(s)

Keywords

Enterococus faecalis

Confocal Laser Scanning Microscopy

N-Acetylcysteine

Photodynamic Therapy

Scanning Electron Microscopy

Abstract

Introduction: This study aimed to evaluate the effectiveness of N-Acetylcysteine (NAC), Photodynamic Therapy (PDT) and NAC with supplemental PDT in optimizing the removal of bacteria from infected dentinal tubules of root canals infected with Enterococcus (E. ) faecalis biofilm. Methods and Materials: Eighty human teeth were randomly divided into 5 groups (n=16) according to the intracanal medication used: saline solution (control); calcium hydroxide (CH); NAC; PDT; NAC+PDT. Ten samples from each group were prepared for microbiological culture analysis (CFU/mL) and were inoculated with E. faecalis suspension for 21 days for biofilm development; the other six samples from each group were prepared for Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM) and submitted to a 5-days contamination protocol including eight centrifugation cycles on every other day for dentinal tubules infection. For antimicrobial activity analysis by microbiological culture, the root canals were contaminated with E. faecalis biofilm, instrumented and then medicated according to the experimental groups. Three samples were collected from the root canals: after 21-days of contamination, immediately after the instrumentation and 14-days after the medication according to the experimental groups. The morphology of E. faecalis biofilm on the root canal walls and bacterial cells viability were assessed by means of SEM and CLSM, respectively. One-way ANOVA and Repeated Measures ANOVA tests were used to analyze the obtained data statistically. Results: CFU/mL analysis showed that CH, NAC and NAC+PDT promoted greater antibacterial activity with statistically significant difference compared to saline solution and PDT (P<0. 0001). However, saline solution and PDT were statistically similar. Illustrative images by SEM confirmed partially the CFU/mL results. CLSM showed that all groups were effective eliminating E. faecalis except for the saline solution group. Conclusions: Based on this in vitro study NAC was bactericidal against E. faecalis biofilms regardless PDT stimulation, presenting similar antimicrobial activity to CH.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

Hasna, Amjad Abu, Khoury, Rayana Duarte, Toia, Cassia Cestari, Goncalves, Glaucia Beatriz, de Andrade, Flaviana Bombarda, Talge Carvalho, Claudio Antonio, Ribeiro Camargo, Carlos Henrique, & Valera, Marcia Carneiro. (2020). In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis. IRANIAN ENDODONTIC JOURNAL (IEJ), 15(4), 236-245. SID. https://sid.ir/paper/984739/en

Vancouver: Copy

Hasna Amjad Abu, Khoury Rayana Duarte, Toia Cassia Cestari, Goncalves Glaucia Beatriz, de Andrade Flaviana Bombarda, Talge Carvalho Claudio Antonio, Ribeiro Camargo Carlos Henrique, Valera Marcia Carneiro. In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis. IRANIAN ENDODONTIC JOURNAL (IEJ)[Internet]. 2020;15(4):236-245. Available from: https://sid.ir/paper/984739/en

IEEE: Copy

Amjad Abu Hasna, Rayana Duarte Khoury, Cassia Cestari Toia, Glaucia Beatriz Goncalves, Flaviana Bombarda de Andrade, Claudio Antonio Talge Carvalho, Carlos Henrique Ribeiro Camargo, and Marcia Carneiro Valera, “In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis,” IRANIAN ENDODONTIC JOURNAL (IEJ), vol. 15, no. 4, pp. 236–245, 2020, [Online]. Available: https://sid.ir/paper/984739/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops