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Information Journal Paper

Title

First Record of HAdV-D20 Among Keratoconjunctivitis Patients in Iraq

Pages

  176-181

Abstract

 Background: Human Adenovirus species D (HAdV-D) was common human viral pathogen especially in eye infection, consists of several types of which HAdV-D8,-D19 and –, D37 were common in eye infection. This study includes detection of HAdV-D types implicated in Conjunctivitis based on L2 (Penton protein) gene similarity. Methods: Conjunctival swabs were collected from KeratoConjunctivitis patients as eye infection related to adenovirus. Viral nucleic acids were extracted and specific primer pairs for HAdV-D L2 gene (encoding for penton base protein) was used to amplify the target gene and only positive samples were sent to sequencing. Results: The results revealed that only 6 samples give positive results for L2 gene amplification and then sent for sequencing for L2 (penton protein) gene-based typing. The results show that 4 local isolates (S1, S2, S3, S6) were similar to HAdV-D8 and 2 local isolates (S4, S5) were similar to HAdVD20. Also the results display that the HAdV-37, prominent HAdV-D type of human eye infection, may be variant of HAdV-D20 due to that six variation were seen in S4and S5 local isolates nucleotide sequence in relation to HAdV-D37: T>C at position 14364, A>C at position 14411, T>C at position 14427, C>A at position 14448, G>A at position 14540 and T>C at position 14617, leading to only 2 amino acid change in resulted penton protein: T (Threonine) instead of K (Lysine) at position 204 and N (Asparagine) instead of D (Aspartic acid) at position 247. Conclusions: The current study concludes the possibility of implication of HAdV-D20 in eye infections especially Conjunctivitis.

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    APA: Copy

    Rashid Al Mousawi, Masar Riyadh, Mohammed Ali, May, Kadhim Al Khafaji, Noor Salman, & Oleiwi Muttaleb Al Dahmoshi, Hussein. (2022). First Record of HAdV-D20 Among Keratoconjunctivitis Patients in Iraq. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 11(1), 176-181. SID. https://sid.ir/paper/987512/en

    Vancouver: Copy

    Rashid Al Mousawi Masar Riyadh, Mohammed Ali May, Kadhim Al Khafaji Noor Salman, Oleiwi Muttaleb Al Dahmoshi Hussein. First Record of HAdV-D20 Among Keratoconjunctivitis Patients in Iraq. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2022;11(1):176-181. Available from: https://sid.ir/paper/987512/en

    IEEE: Copy

    Masar Riyadh Rashid Al Mousawi, May Mohammed Ali, Noor Salman Kadhim Al Khafaji, and Hussein Oleiwi Muttaleb Al Dahmoshi, “First Record of HAdV-D20 Among Keratoconjunctivitis Patients in Iraq,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 11, no. 1, pp. 176–181, 2022, [Online]. Available: https://sid.ir/paper/987512/en

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