Methylation of the PKD1 Promoter Inversely Correlates with its Expression in Autosomal Dominant Polycystic Kidney Disease

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Hajirezaei Fatemeh; GHADERIAN SAYYED MOHAMMAD HOSSEIN; HASANZAD MANDANA; NAFAR MOHSEN; GHADIANI MOHAMMAD HASSAN; Biglari Sajjad; Sohrabifar Nasim; JAFARI HOSSEIN

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Journal: REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY Year:2020 | Volume:9 | Issue:2 Page(s): 193-198

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Title

Methylation of the PKD1 Promoter Inversely Correlates with its Expression in Autosomal Dominant Polycystic Kidney Disease

Author(s)

Keywords

Autosomal Dominant Polycystic Kidney Disease (ADPKD)

Epigenetic

Methylation

methylation-sensitive high-resolution melt (MS-HRM) analysis

PKD1

Abstract

Background: Autosomal dominant polycystic kidney disease (ADPKD), a multisystem disorder, is the most prevalent type of hereditary kidney disease. Here, we aimed to evaluate Methylation of the PKD1 gene (PKD1) promoter and its correlation with PKD1 expression in peripheral blood. Methods: In this case-control study Methylation of the PKD1 promoter was evaluated using Methylation-sensitive high-resolution melt (MS-HRM) analysis. PKD1 expression was assessed by quantitative real-time PCR. The correlation was evaluated using the Pearson correlation test. Results: Twenty subjects from both the patient and control groups (n= 40 for each) were methylated at the PKD1 promoter to various levels (18. 9% in patients and 62. 5% in controls). This difference was statistically significant (p< 0. 0001). PKD1 expression in blood samples was significantly greater in ADPKD patients than in controls (p= 0. 0081). Significant correlation was seen between PKD1 expression and its promoter Methylation status in peripheral blood (r case=-0. 5300, p= 0. 0162, and r control =-0. 6265, p= 0. 0031). Conclusions: Methylation of the PKD1 promoter in ADPKD patients was inversely correlated with PKD1 expression.

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APA: Copy

Hajirezaei, Fatemeh, GHADERIAN, SAYYED MOHAMMAD HOSSEIN, HASANZAD, MANDANA, NAFAR, MOHSEN, GHADIANI, MOHAMMAD HASSAN, Biglari, Sajjad, Sohrabifar, Nasim, & JAFARI, HOSSEIN. (2020). Methylation of the PKD1 Promoter Inversely Correlates with its Expression in Autosomal Dominant Polycystic Kidney Disease. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 9(2), 193-198. SID. https://sid.ir/paper/989402/en

Vancouver: Copy

Hajirezaei Fatemeh, GHADERIAN SAYYED MOHAMMAD HOSSEIN, HASANZAD MANDANA, NAFAR MOHSEN, GHADIANI MOHAMMAD HASSAN, Biglari Sajjad, Sohrabifar Nasim, JAFARI HOSSEIN. Methylation of the PKD1 Promoter Inversely Correlates with its Expression in Autosomal Dominant Polycystic Kidney Disease. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2020;9(2):193-198. Available from: https://sid.ir/paper/989402/en

IEEE: Copy

Fatemeh Hajirezaei, SAYYED MOHAMMAD HOSSEIN GHADERIAN, MANDANA HASANZAD, MOHSEN NAFAR, MOHAMMAD HASSAN GHADIANI, Sajjad Biglari, Nasim Sohrabifar, and HOSSEIN JAFARI, “Methylation of the PKD1 Promoter Inversely Correlates with its Expression in Autosomal Dominant Polycystic Kidney Disease,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 9, no. 2, pp. 193–198, 2020, [Online]. Available: https://sid.ir/paper/989402/en

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