Dog rose (Rosa canina L.) is considered as one of the most important medicinal plants, which contain valuable medicinal and nutritional compounds in its fruits. In order to determine total phenolic, soluble carbohydrates, carotenoid contents and minerals in dog rose fruits collected from five different ecological regions in south-west of Iran (Kiar and Gerd Bishe in Charmahal & Bakhtiari, Yasuj and Meymand in Kohgiluye & Boyer Ahmad and Semirom in Isfahan), an evaluation was carried out during 2006-2007. Total phenolic contents, total soluble carbohydrates and total carotenoids varied from 83.13-94.14 mg GAE/g DW, 13.34–17.14% and 408.36–495 µg/g FW, respectively. The N, P, K, Mg, Ca, Fe, Zn and Mn values of fruit samples differed from 0.73-1.15%, 2816–4278 ppm, 2036–3325 ppm, 689–1092 ppm, 821–1243 ppm, 34–52 ppm, 15–29 ppm and 23–43 ppm, respectively. This study showed that fruits of dog rose are a rich source of phenolic contents, soluble carbohydrates, carotenoids and minerals. In this study, content of active substances affected by climatic factors of case studies was investigated.