Introduction: Ovary cancer is one of the commonest cancers among the women. With regard to role of cyclooxygnase (COX) enzyme and production of prostaglandin type E2 in causing tumor damages in ovary cancer, application of compounds to inhibit cyclooxygnase can be effective in preventing ovary cancer. Thus, the present study was carried out to evaluate FLUNIXIN as nonselective inhibitor of Cyclooxygenase enzymes in developing ovary cancer in female Wistar rats.Methods: In this experimental research, forty eight female Wistar rats, assigned into six groups, were used. To induce cancer, 7, 12 Dimethyl Benza Anthracene was directly injected into ovary. Control groups included a negative control group (no injection), a DMBA injection control group and a positive control group (DMBA+Saline injection). Ten days after administrating DMBA to the tumor, three doses (0.5, 1, 1.5 mg) of FLUNIXIN meglumine were injected into the three case groups every third day. The comparison between groups was made by using one-way ANOVA.Results: The mean weight of tumors significantly decreased in drug receiving groups, compared to positive control and DMBA control groups. The mean weight of ovary in the three groups receiving FLUNIXIN did not show any significant difference, compared with the negative control (natural) group (P>0.05).Conclusion: This study confirms the positive effect of the FLUNIXIN in treating the ovary cancer. The microscopic slices of ovary tissues in the experimental (drug receiving) groups indicate that cancerous cells are decreasing so that the cells tend to gain their own normal order again.

Introduction: FLUNIXIN Meglumine (FM) is a nonsteroidal antiinflammatory drug (NSAID) and nonselective cyclooxygenase (COX) inhibitor. Since it has been suggested that COX enzyme plays a role in seizure, this research is performed to assess the therapeutic effects that this compound might have in seizures induced by PTZ.Methods: In this research, male wistar rats (200 ± 20 g) were given intracerebroventricular injections of saline or FM (12.5 mg, 25 mg and 50 mg) in a 1 ml volume, before intraperitoneal administration of PTZ (80 mg/kg) for induction of seizure. Then, seizure score and times of onset for every stage of seizure were recorded during 20 minutes after PTZ administration. The data were analyzed by one-way analysis of variance (ANOVA).Results: injection of 25 and 50 mg of FM significantly increased times of onset of every seizure stage compared to the control group. The group that received 50 mg of FM did not show stage 5. On the other hand, 12.5 mg FM group did not show any significant difference with the control group.Conclusion: We conclude that FM has dose dependent anticonvulsive properties.

Background: Nutmeg, Myristica fragrans Houtt, has shown anti-inflammatory properties in some studies. At present experimental study, we evaluated the effect of seed extract of nutmeg on adjuvant-induced arthritis in rats in comparison with FLUNIXIN meglumine.Materials and Methods: Experimental study was done in six groups of Wistar rats (each group 8 rats) as following: Group 1 was kept as control under similar conditions to other groups. All other rats received complete Freund's adjuvant at dose 0.1 ml which injected under skin of foot. Group 2 was received vehicle (normal saline). Group 3 received FLUNIXIN intraperitonealy at dose of 2 mg/kg body weight of rats daily for 12 days. Group 4 to 6 received extract of nutmeg at dose 100, 200 and 300 mg/kg intraperitonealy and daily for 12 days. Four rats in each group were anesthetized and blood collected for serum analysis on 12th day. The ankle joint prepared for histopathological examination. The remained rats were kept until 21th day. Levels of the cytokine TNF-a in serum was measured using ELISA kit.Results: The serum levels of TNF-a in the group 2 were significantly increased; while nutmeg decreased the elevated TNF-a level in a dose-dependent manner but significantly with 300 mg/kg. The FLUNIXIN did not significantly decrease the levels of TNF-a. Nutmeg treated rats manifested pathological events in the ankle joints to a markedly lesser degree. FLUNIXIN prevented pannus formation but it was ineffective in other lesions.Conclusion: Thus, nutmeg protected the joints against cartilage destruction and bone erosion in a dose-dependent manner.

The objective of this study was to examine the effects of administration of FLUNIXIN Meglumine on conception rates and plasma progesterone (P4) changes in Holstein heifers and inhibition of PGF2a synthesis just before luteolysis. 32 Holstein heifers (15-month-old, BW: 357±5kg) Were Synchronized utilizing Ovsynch protocol and artificially inseminated. The heifers were assigned randomly to a treatment group (n=16) and a control group (n=16). The treatment groups were injected intramuscularly three times on days 15.5, 16 and 16.5 post insemination with 1.1 mg/kg bodyweight FLUNIXIN Meglumine whereas control group received placebo. Blood samples were collected on days 15.5, 16 and 16.5 post insemination for analysis of plasma P4 concentrations. Pregnancy was diagnosed on days 30 and 60 by transrectal ultrasonography. On day 30, 10 of the treated heifers were pregnant compared with 4 of the control heifers (P<0.05); on day 60, 9 of the treated heifers were still pregnant compared with 4 of the control heifers (P>0.05). Mean plasma progesterone concentrations on day 16.5 in treated heifers (2.62±0.66 ng/ml-1) was higher than control group (1.87±1 ng/ml-1) but this improvement was not statistically significant (P>0.1). The results of this study support the hypothesis that FLUNIXIN Meglumine is able to inhibit the uterine synthesis of PGF2a and increase the survival of early embryos.

Aim and Background: Based on existing scientific information Thymus vulgaris is a medical herb which has anti- inflammatory effects. Visceral pain is an unpleasant feeling, however its treatment with NSAIDs results in serious side effects such as gastric ulcer, blood dyscrasia and Renal failure. In order to decrease these side effects and the dosage of drugs, co-administration of synergistic herbal medicine with these drugs can be a solution. The aim of this study was to evaluate the palliative interactive effect of hydroalcholic extract of Thymus vulgaris and FLUNIXINmeglumine on visceral pain.Methods and Materials: This experimental study was performed on 40 N-MRI male mice (36±4g).Animals were randomly divided into five groups: Negative Control group treated with normal saline, Positive Control group treated with FLUNIXIN (2 mg/kg) and treatment groups receiving FLUNIXIN (1mg/kg), Thymus vulgaris (100mg/kg) and both Thymus (100mg/kg) plus FLUNIXIN (1mg/kg). All these groups received drugs and hydroalcholic extracts via intraperitonial injection. The Mices were injected with acetic acid 0.6% (10ml/kg) for visceral pain induction, and 15 minutes after each intraperitoneal administration, palliative effects were recorded by counting the number of Writhing during 30 minutes. The data was analyzed by SPSS using One-Way ANOVA (Tukey) test. The significant value was shown with p<0.001.Findings: The positive control group and treatment groups showed a significant reduction in pain response when compared to negative control group (p<0.001). Both Postive control and Thymus-FLUNIXIN groups had a significant reduction in pain response in comparison with both FLUNIXIN and Thymus groups (p<0.001) but they showed no significant difference relative to each other (p>0.001).Conclusions: Co-administration of Thymus vulgaris and FLUNIXIN results in synergistic analgesic effect.

Background and objectives: Non steroidal anti inflammatory drugs (NSAIDs) are widely used to reduce inflammation, pain and fever. The present study was organized to induce an experimental inflammation in an animal model, using a putative biological oxidant, Peroxynitrite (ONOO-) and to study the effects of FLUNIXIN meglumine and Ketoprofen on the pressure-induced pain.Materials and Methods: For this purpose, 24 male guinea pigs were divided into 4 groups each consisting of 6 animals. Three groups (groups 2, 3 and 4) were injected Peroxynitrite and one group (the first: control) received physiological salt solution subcutaneously in the paw. After induction of a local inflammatory response, FLUNIXIN meglumine (1mg/kg) and Ketoprofen (2 mg/kg) were injected to the second and third groups, 5 times with 12h intervals. The first and the fourth groups were injected saline solution in the same manner. Pressure analgesiometry was performed before and 1 hour after injections. Result: The animals in all 3 groups treated with Peroxynitrite demonstrated an increased sensitivity to painful pressure (P<0.05). Both NSAIDs decreased the pain sensation dramatically after the 1st and the 2nd injections but, not after the 3rd, 4th and the 5th injections.Conclusion: The study suggested that NSAIDs may be helpful in reducing pressure-induced pain in animal model in early hours of the treatment, whereas the effect subsides over time and ends up after a few days. This effect may be of importance in humans who receive these kinds of drugs for a long period as they may not be effective in reducing pain after a while.

Background: Ovarian cancer is the deadliest gynecologic cancer. Studiesonthe therapeutic properties of Ginkgo bilobaand FLUNIXIN showed that these drugs, singly or in combination with other drugs, have anti-cancer activities. Different genes are involved in apoptosis regulation. The BIM gene is one of the most important regulators of this process. BIM has different roles, including cell cycle regulation, apoptosis induction, deoxyribonucleic acid recombination, chromosomal segregation, and cell aging. Methods: This study evaluated the viability percentage of the A2780s cell line with Ginkgo biloba and FLUNIXIN at different concentrations, compared to that of the control group. Then, the half-maximal inhibitory concentration (IC50) values of Ginkgo biloba and FLUNIXIN were determined within 24 h. Then, the expression of the BIM gene was evaluated using a real-time polymerase chain reaction (PCR). Results: The IC50 results showed that Ginkgo biloba and FLUNIXIN significantly reduced cell life (P < 0. 01) depending on time and concentration. The results of real-time PCR showed that cell treatment with Ginkgo biloba and FLUNIXIN significantly increased BIM expression. Conclusions: The results of this experiment indicated that BIM gene expression was increased in cancer cells treated with Ginkgo biloba and FLUNIXIN, compared to that reported for control cells. Therefore, with further research in the future, these compounds can be used for the development of ovarian anti-cancer drugs.