In the present study LHRHa2 was tested along with Metoclopramide and Chlorpromazine on 52 pieces of Abramis brama orientalis in May 2008. In order to identify the optimum doses which were proved to be (1, 2, 3, 4 mg/kg) for LHRHa, (5, 10, 15, 20 mg/kg) for Metoclopramide and (2, 5, 10, 12 mg/kg) for Chlorpromazine. The treatment sample fish were classified into five categories, and then the injected solvents were prepared. The procedures of the study included injection operations into the muscles and cupping was in the form of sagittarius hemal. After that, GTH II was conducted on the basis of RIA. The findings proved that all the compositions in a stimulating process resulted in an increase in the ration of GTH II and at P<0.05 resulted in meaningful differences in optimum dose. LHRHa2 (3 mg/kg) led to 0.554 IU/L, Metoclopramide (5 mg/kg) caused 0.618 IU/L and also Chlorpromazine (10 mg/kg) resulted in 0.522 IU/L increase in the ratio of GTH II.