Background and Objectives: The main purpose of this research is to investigate the performance of Knowledge Based Companies in Southern, Northern, and Razavi Khorasan provinces based on the performance prism model. The results of this research provide solutions to improve the performance of new Companies, especially knowledge-based Companies, as a " pioneer Institution in acquiring value-added in the knowledge economy Method: The present research is purposeful, applied and field survey type. Data gathering tool in this research, were used a researcher-made questionnaire based on the theoretical model of the performance prism of Nilly et al. (2014) consisting of 5 components. The statistical population of this study included all employees of the knowledge based companies is located in Science and Technology Park of South, North and Razavi Khorasan provinces in 2019. The statistical sample was determined based on the formula of the number of indices in the structural equations also basis of programming in software (R) and with at least 80% of the test power and the amount (RMESEA), which were 140 person. Findings: The results of the CFA analysis have shown that the five components of the performance prism in knowledge-based companies (such as key stakeholders satisfaction, key stakeholders strategies, critical processes and key processes, capabilities, assistance Key stakeholders) as well as its indicators to measure the performance of knowledge-based firms are suitable Conclusion: Among the five components of the performance prism, stakeholder contribution with 3. 13 value has the largest average of the other components, although this component still has a fundamental gap to reach the top position, and the component of strategies with a value of 2. 85 is the smallest that knowledge-based companies in Khorasan provinces have been weak in strategies. Also, the average performance is 3. 2, which is moderate, indicates that Knowledge-based companies need to invest more in this area in order to fulfill their capacities and potentials in the Khorasan provinces. In this regard, proper policy making and support for them and provision infrastructure for their effective presence, especially in international markets, will be important.