In order to investigate mustard effect on seed yield and seed oil and protein content, an experiment with factorial arrangement within a randomized complete block with four replications was conducted in 2007 at field research of Mollasani Agricultural Extension Center, Ahvaz, Iran. Treatments included, mustard density at five densities (0, 7, 14, 21 and 35 plants m-2) as primary factor and canola density at three densities (60, 80 and 100 plants m-2) as secondary factor. The results indicated that seed yield, oil percentage, oil yield and protein yield of seed canola were significant affected by interaction between canola and mustard densities, but protein percentage of seed canola wasn’t significantly. the increase in mustard density at various densities of canola caused to reduction in seed yield (87, 89 and 80 percentage decreasing in 60, 80 and 100 plants of canola, respectively), oil percentage (26, 47 and 52 percentage decreasing in 60, 80 and 100 plants of canola, respectively), oil yield (90, 94 and 90 percentage decreasing in 60, 80 and 100 plants of canola, respectively) and protein yield (85, 86 and 75 percentage decreasing in 60, 80 and 100 plants of canola, respectively). In addition, enhanced canola density increased oil percentage, while the highest oil yield was obtained in 80, 100 and 60 plants of canola, respectively. Protein and seed yield had a similar to change pattern in various density of canola. The highest protein and seed yield observed by 80, 100 and 60 canola plants, respectively. In addition, enhanced mustard density decreased seed yield and oil percentage, but was differ this decreasing at various density of canola. While was opposite protein percentage.