In order to study the effect of silicon on biochemical traits, leaf relative water content, and yield of two bread and durum wheat cultivars under late season water stress conditions, a split factorial experiment have been conducted in a randomized complete block design in three replicates at College of Agriculture and Natural resources of Darab, during 2017-2018 growing season. The treatments include water stress in two levels, i. e., normal irrigation and water stress at the end of flowering, silicon (Si) spraying at 0, 1, 2, and 3 mM, and two wheat cultivars: Chamran as bread wheat and Shabrang as durum wheat. Results show that the main effects of late seasonal water stress, cultivar and silicon on leaf relative water content (RWC), total chlorophyll, carotenoid content, and yield have been significant. Under water stress conditions, when the plants are exposed to 3 mM silicon, RWC rises by 50%, compared to no silicon application conditions. Grain protein under water stress conditions and 3 mM silicon was 59. 3% higher than no silicon condition. Also, total chlorophyll content and carotenoid content under water stress conditions and application of 3 mM silicon have increased by 42. 5% and 44. 9%, respectively. In Chamran cultivar, application of silicon at 3 mM has raised total chlorophyll content, carotenoid content, RWC, and grain protein by 40%, 43%, 42%, and 56. 4%, respectively, causing a 19. 7% increase in grain yield, compared to Shabrang cultivar under water stress conditions. Calculation of correlation coefficients between grain yield and other traits shows that under normal irrigation conditions, the highest correlation could be observed between grain yield with total chlorophyll (r =0. 815 **) and number of spike per square meter (r =0. 814**) and under stress conditions between grain yield with number of spike per square meter (r =0. 937**) and total chlorophyll (r = 0. 840**). In general, by improving total chlorophyll content, carotenoid content, and RWC, foliar application of 3 mM silicon can play an important role in increasing grain yield, especially under water stress conditions.