Journal of Veterinary Research
Year:2019 | Volume:74 | Issue:3 |Papers Count:17

Nowadays, with the development of new technologies, improved and progressed methods have been taken to diagnose, treat and prevent cancers. Pathologic study and some molecular methods have been helpful in diagnosing and predicting cancer but these methods are not enough in many cases. Omics technology investigates many parts of cells such as genes, proteins, transcripts, and metabolites simultaneously. This procedure provides a more real and general feature of cellular processes, especially in cancer cells. In human, Omics technology is widely used to diagnose and treat various cancers and predict prognosis of tumors and survival of patients. In parallel to the studies of cancers in human, similar investigations were conducted in the canine cancers. Regarding the importance of Omics method in oncology, we described various Omics techniques including genomics, transcriptomics, and proteoimcs. In addition, corresponding studies carried out in different canine cancers were summarized in the next step.

BACKGROUND: Toxoplasma gondii is a zoonotic obligate intracellular protozoan parasite that infects all warm-blooded animals as well as human worldwide. Human is infected with Toxoplasma parasites by eating half-cooked meat of livestock or oocyte. OBJECTIVES: The purpose of this study was to determine the presence of Toxoplasma in aborted fetuses of sheep using the serological method in North Khorasan province. METHODS: In this study, from 2015 to 2017, 133 samples of the thoracic fluid in aborted fetuses of sheep from different cities of North Khorasan Province were collected and sent to the central laboratory of Bojnourd. For each sample, a questionnaire was prepared for gathering information such as age and city, and then, the antibody level was determined in each sample by ELISA method. RESULTS: In this study, the age of 133 aborted fetuses of sheep was more than 120 days. Also, of the 133 aborted fetuses of sheep, 14 samples (10. 53%) were infected with Toxoplasma parasites. The highest and lowest rate of Toxoplasma infection was observed in 2016 and 2017, respectively. Also, the most infection was found in Shirvan and Faroj cities. Results of chi-square test showed that there was a significant difference between year and abortion in sheep due to infection with Toxoplasma parasite (P<0. 05). There was not significant difference between the frequency of this parasite infection and aborted fetuses in different areas (P> 0. 05). CONCLUSIONS: The results of this study indicate that Toxoplasma gondii infection is one of the causes of abortion of sheep in North Khorasan province, and sheep is one of important sources of meat and dairy products in this area, Therefore, observance of public health tips and the complete cooking of meat and boiling milk is emphasized.

BACKGROUND: Hydatid cyst is an infection with global distribution that is caused by the larval stage of the tapeworm of Echinococcus. The long-term survival of the hydatid in the host shows the parasite has advanced highly effective strategies for escaping the host defense. Deaths are caused by parasitic infections which are often due to tissue damages that result in host cell death, this is known as apoptosis. So it is important to know the process and the role of apoptosis that is created or controlled by a parasite. OBJECTIVES: Investigation of cytotoxicity effect, induction of apoptosis and mechanism of induction of apoptosis of cattle hydatid fluid on bovine lymphocyte cells as efficient cells of immunity were studied. METHODS: In this study, the cytotoxicity effect of bovine hydatid fluid (HF) on lymphocyte cells was investigated as effective immune cells against Echinococcus granulosus by MTS method. Then the mean of the expression of caspase-3, Bax and Bcl-2 genes in bovine lymphocytes treated/untreated was determined with fertile and infertile hydatid cyst fluid using Real Time PCR method. RESULTS: The viability mean of lymphocytes was significantly lower in the fertile HF treated lymphocytes compared to both infertile HF-treated lymphocytes and cell control. Bax gene expression was significantly (P=0. 046) higher in the fertile HF-treated lymphocytes compared to both infertile HF-treated lymphocytes and cell control. Although Caspase 3 was higher in this group, the difference was not significant. Also, expression of Bcl-2 gene in fertile fluid treated lymphocytes was found to be lower than that of infertile and control. CONCLUSIONS: Present study indicates that hydatid cyst fluid molecules can probably induce apoptosis in immune cells in vitro and the parasite’ s ability to stay alive for a long time in the host by controlling the host immune response from the apoptosis pathway.

BACKGROUND: Ticks are important ectoparasites in ruminants which cause economic losses in animal husbandry of Iran and worldwide. OBJECTIVES: This study was aimed to determine geographic distribution, frequency and species diversity of hard ticks in domestic ruminants in Ilam province, Iran. METHODS: A total of 445 domestic ruminants (139 cattle, 162 sheep, 144 goats) from 120 flocks of 30 villages in north and south parts of Ilam province were randomly selected and examined in summer 2015. The ixodid ticks were collected from body surface of examined animals and identified. RESULTS: Of all examined ruminants, 44. 6% cattle, 51. 23% sheep, and 52. 08% goats were infested with a total number of 1209 unfed ixodid ticks. The highest prevalence of hard ticks was found in 2-3 year-old female cattle (15. 55%), 1-2 year-old female sheep (19. 75%) and less than 1 year-old female goats (17. 36%) in the region. The highest prevalence was found in 2-3 year-old female cattle (37. 1%), 1-2 year-old ewes (38. 55%) and less than 1 year-old kids (33. 33%). There was significant difference between prevalence and different age groups of infested sheep and cattle. Of 1209 collected ixodid ticks, tick indices (tick number per animal) were 6. 1, 5. 9 and 4. 5 in cattle, sheep, and goats, respectively. The highest infestation in cattle (17 flocks, 14. 1%), sheep (13 flocks, 10. 8%) and goats (14 flocks, 11. 6%) was respectively found in north, south and southern parts of the region. The highest tick aggregation was found for ears in cattle (31. 13%), sheep (34. 41%) and goats (28. 9%). Of all examined ticks (1209), two genera including Hyalomma (37. 62%) and Rhipicephalus (62. 38%) with seven, seven, and six species in cattle, sheep, and goats were respectively identified. The predominant infesting ticks were R. sanguineus (22. 16% in cattle) from north (11. 31%) and H. anatolicum anatulicum (21. 8% in sheep and 24. 77% in goats) from south (15. 49% and 13. 42%) part of the province. CONCLUSIONS: The results revealed that species diversity and frequency of ixodid ticks were prevalent in domestic ruminants of different parts of Ilam province.

BACKGROUND: Parasitic helminthic diseases are one of the most common diseases of humans and animals that threaten the health of human societies. Nowadays, different methods are used for recovery of eggs parasites from soil samples. Therefore, in order to reduce the amount of mistakes caused by the artifacts (waste and disposable materials for diagnostic purposes), it is necessary to design and use a special device to collect more accurately the eggs of helminthic parasites. OBJECTIVES: The purpose of this study was to design and use a rapid method for collecting different Toxocara spp. eggs in order to determine the prevalence of soil contamination. METHODS: In this study, for recovery of Toxocara parasite eggs from collected soil samples, a sieve screen was used with plastic body (polyvinyl chloride compressed plastics) in a cylindrical shape with a mesh of 150 µ m (can be changed), as well as the cap and holder, for the use of modified sucrose flotation method to isolate and identify Toxocara species eggs. RESULTS: In the current study, single cell, multicellular, and infective Toxocara eggs were recovered from collected soil samples. The results of prevalence of Toxocara eggs in collected soil specimens showed that 38. 5% (CI: 95%, 32. 03-45. 40%) were recovered using vertical sieve screening and using the traditional technique and flotation method 21. 5% (CI: 95%, 16. 37-27. 70%) were recovered, which showed a significant difference between the two groups (P˂ 0. 05). CONCLUSIONS: Compared to the conventional and standard sucrose flotation method, using the sieve screen in addition to recovery of Toxocara parasite, it can be used in epidemiological studies to investigate the presence of eggs of nematode parasites such as Ascaris lumbricoides and Trichocephalus, as well as other eggs of zoonotic helminths in soil samples, and the percentage of true egg parasites in the soil samples in epidemiological studies.

BACKGROUND: Due to the incomplete microbial population of the gastrointestinal tract in infant animals, the occurrence of any kind of stress causes gastrointestinal microbial imbalance and gastrointestinal disorders in the animal. The use of probiotics in diets of experimental animals improves the production of volatile fatty acids in rumen as the main stimulants of ruminal papillae. In this case the beneficial microbial population promotes in the rumen and its products improve the health and animal performance. OBJECTIVES: The aim of this study was to investigate the effects of different levels of Protexin™ probiotics in milk replacer on digestive tract development and ruminal parameters of suckling Zel lambs. METHODS: To conduct this experiment, 24 male lambs were used at 10 days of age with mean weight (4. 2 ± 0. 53 kg) in 4 treatments and 6 replicates per each treatment as individual pens for 60 days. Treatments include control (without probiotic) and 3, 6 and 9 g (×109 cfu/g) of probiotic in milk replacer. In day 60 of experiments, pH values were measured by a portable digital device and ammonia nitrogen and number of protozoa in rumen fluid were measured in laboratory. Ruminal morphological results were studied after animal was slaughtered. RESULTS: The results of ruminal parameters showed that adding 9 g probiotic resulted in a significant increase in ammonia nitrogen and number of ruminal fluid protozoa compared to other levels (P<0. 05). There were not significant differences in pH and VFA of ruminal fluid indicated no significant difference among treatments. In rumen morphology results, there was a significant difference between treatments in weight of empty whole digestive tract, rumen, omasum and abomasums weight and the volume of reticulum and abomasum (P<0. 05) and this difference was significant between treatment of 9 g probiotic and other treatments. Also, the effect of experimental treatments was significant on length and width of the rumen papillae (P<0. 05), so that level of 9 g probiotic was higher than other levels. CONCLUSIONS: The results showed that the addition of probiotic in milk replacer of experimental lambs significantly increased ruminal VFA and developed the ruminal morphological traits, so that in these traits, amount of 9 g probiotic had better performance than the other levels.

BACKGROUND: Although numerous reports support the beneficial effects of supplementing chromium (Cr) on broiler performance under normal and stress conditions, the optimal level has not been determined yet. OBJECTIVES: This study was conducted to investigate the effects of different levels of supplemental Cr on performance, cortisol, and thyroid hormones of broiler under normal and stress conditions. METHODS: A total of four hundred forty-eight broilers were used. Broilers allocated into 2×4 factorial experiment included: stress (normal and stress) and 4 levels of supplemental chromium (0, 1000, 2000, 3000 ppb) since day 18. A completely randomized design with 8 treatments and 4 replicates (14 birds per replicate) was used. Dexamethasone used as a stressor for one week (stress period, STP) then experiment continued to day 46 (recovery period, RCP). RESULTS: Feeding 1000 and 2000 ppb Cr improved feed conversion ratio (FCR) and increased T4 compared to 0 and 3000 ppb Cr in stressed broilers in STP (P<0. 05). Feed intake (FI), body weight (BW) and T3 concentration were higher in broilers fed with 1000 ppb Cr in the diet and reared without stress in STP (P< 0. 05). Despite the negative effect of stress on performance in RCP, dexamethasone-stressed broilers had better FCR. Feeding stressed birds with 1000 ppb Cr increased blood cortisol whereas it reduced cortisol in the normal-reared birds. CONCLUSIONS: The Dietary supplementation of 1000 and 2000 ppb Cr reduced deleterious effects of physiological stress. Moreover, 1000 ppb Cr improved FI and BW of broilers under normal condition at first week. Feeding high level of chromium under normal and stress conditions did not improve the performance.

BACKGROUND: Zearalenone (ZEA) is a nonsteroidal estrogenic mycotoxin that is usually found in animal feed and causes disorder in genital organs activity. Most commercial adsorbents do not have ZEA absorbency and may have side effects on the animal performance. Therefore, the discovery and introduction of natural compounds are necessary to reduce ZEA. OBJECTIVES: The introduction of some medicinal plants to degrade ZEA in rumen fluid is the main objective of this study. METHODS: In the present study, essential oil and different extracts (methanol, n-hexane and ethyl-acetate) of seed of four medicinal plants belonging to Apiaceae family including coriander (Coriandrum sativum), Cumin (Cuminum cyminum), Fennel (Foeniculum vulgare) and Persian hogweed (Heracleum persicum) were investigated to reduce ZEA in rumen fluid (0. 4µ g ZEA in ml 20% rumen fluid) at the ratio of essential oil/extract to toxin 125: 1, 250: 1 and 500: 1 in 48h. The ZEA-content was extracted by the immunoaffinity column and analyzed by high-performance liquid chromatography (HPLC-FLD). RESULTS: The results showed that essential oil of coriander (contains 76. 5% of linalool), n-hexane extract of coriander and methanol and ethyl acetate extracts of Persian hogweed exhibit acceptable efficiency (more than 30%) in ZEA degradation. ZEA evaluation in the presence of various concentrations of promising essential oils and extracts exhibited that the essential oil of coriander has the highest effect to remove ZEA from rumen fluid with 79. 5% after 48 h. The n-hexane extract of coriander at the rate of 500: 1 caused 67. 8% and 74. 2% reduction in ZEA content after 36 and 48 h incubation time respectively and located at the next statistical level. In addition, methanol and ethyl-acetate extracts of Persian hogweed at the rate of 500: 1 reduced 46% and 41. 8% ZEA content in rumen fluid respectively. CONCLUSIONS: Coriander and Persian hogweed are introduced as promising botanical additive sources to remove ZEA in animal feed.

BACKGROUND: Poultry meat belongs to perishable foods and the major concern of food industries is the microbial spoilage of poultry meat. OBJECTIVES: The aim of present study was to investigate the effect of chitosan (CH) coating enriched with different concentrations of Ziziphora clinopodioides essential oil (ZEO) in comparison with control group on some of the sensory and microbial properties of chicken breast fillets during storage at refrigerated temperature for 12 days. METHODS: Essential oil extraction was done by hydro-distillation method and its chemical composition was determined by gas chromatography with mass spectrometry (GC-MS). In the present study, chicken breast fillets separately were dipped in 2% CH solution containing ZEO at concentrations 0, 0. 5 and 1% and then stored at refrigerated condition for 12 days. After that chicken fillets were studied at 7 intervals (0, 1, 3, 5, 7, 9 and 12 days) regarding microbial (Total mesophilic and Psychrotrophic bacteria, Pseudomonas spp. and Enterobacteriaceae) and sensory (color, odor and taste) examination. RESULTS: The most important compounds of the ZEO were geraniol (20. 62%), carvacrol (18. 17%), thymol (5. 39%), α-terpineol (7. 49%) and 4-terpineol (6. 83%). Results of this study revealed that in the treatments coated with CH containing ZEO, total viable counts (TVC), Pseudomonas spp., lactic acid bacteria, Psychrotrophic bacteria and Enterobacteriaceae familysignificantly (P<0. 05) decreased as compared to control group during the storage period. Based on the results of the present study, coating of chicken fillets with chitosan alone or chitosan containing 0. 5 % concentration of ZEO showed better sensory properties. CONCLUSIONS: CH coating enriched with 0. 5 % ZEO has potential to extend shelf life of chicken fillets without any unfavorable organoleptic properties.

BACKGROUND: Tobacco (Nicotiana tobaccum) is a plant with piscicidal and fertilizing properties. OBJECTIVES: In this study the effect of different concentrations (0. 1, 1, 10 and 100mg/l) of hydro-alcoholic extracts of tobacco has been examined on different tissues of Rainbow trout. METHODS: Samples from liver, kidney and gill of Rainbow trout were stained with hematoxylin and eosin and studied under light microscopy. RESULTS: Various histopathological lesions were observed in the liver of Rainbow trout exposed to different concentrations of tobacco. The results showed that with increase in concentrations of tobacco up to 10 and 100mg/l, bloodshed increased and hepatocytes lost their continuity and showed discontinuity mood. Lamellar aneurysm, bleeding and leukocytes infiltration, clubbing at the end of the lamellae, hyperplasia of secondary lamellar epithelium, epithelial lifting of secondary lamellae and creating an edematous space, lamellar shortening, vacuolation and lamellar fusion were the more abundant branchial lesions after exposure to tobacco. Separating the epithelium from basement membrane, hypertrophy, glomerular capillary dilation and tubular degeneration were observed in renal tissues in all treatments. At higher concentrations of tobacco (10 and 100mg/l), renal tubular necrosis was also observed. According to the results liver of rainbow trout showed a few histopathological alteration following exposure to tobacco extracts. Unlike the liver, gills of rainbow trout due to direct contact with pollutants showed more lesions. CONCLUSIONS: Tobacco extract has more pathological effects on gills in comparison with liver and kidney of Rainbow trout due to direct contact with the dissolved pollutant.

BACKGROUND: Cobalt is an essential nutrient for ruminants which is required for the synthesis of vitamin B12 by the ruminal microflora. Cobalt deficiency can lead to a deficiency of vitamin B12, which plays a major role in many metabolic reactions in the body. OBJECTIVES: This study was conducted to evaluate cobalt status in the serum and liver of cattle. METHODS: In different seasons, the blood serum and liver concentration of cobalt in slaughtered cattle in Urmia city were measured by atomic absorption in 151 and 196 cattle, respectively. RESULTS: The results of cobalt measurement indicated that in 12. 6% of serum and 21. 4% of liver samples the concentration of cobalt was less than normal range with the Mean ± SD value of 0. 64 ± 0. 25 µ g/dl and 0. 15 ± 0. 04 µ g/gDM, respectively. The seasonal variations of cobalt values were assessed in serum and liver samples and following results were obtained: spring (2. 18 ± 1. 64 µ g/dl, 0. 23 ± 0. 13 µ g/gDM), summer (3. 42 ± 1. 04 µ g/dl, 0. 42 ± 0. 27 µ g/gDM), autumn (3. 45 ± 0. 37 µ g/dl, 0. 47 ± 0. 38 µ g/gDM) and winter (2. 75 ± 0. 59 µ g/dl, 0. 35 ± 0. 09 µ g/gDM). CONCLUSIONS: According to the results of this study, it seems that there is a subclinical deficiency of cobalt in cattle of this region and it is recommended that preventive measures be considered to prevent the complications and disorders caused by its clinical deficiency.

BACKGROUND: Virulent Newcastle disease virus (vNDV) imposes significant economic losses to the commercial poultry industry in our country and worldwide. However, in Iran scattered and relatively few studies have been done in order to characterize NDV isolates. OBJECTIVES: The aim of the present study was to characterize two vNDV isolates obtained from commercial poultry farms in Isfahan province in 1999 through Haemagglutinin-Neuraminidase (HN) gene complete sequencing. METHODS: Haemagglutinin-Neuraminidase (HN) gene of each NDV isolate was amplified and sequenced using specific primers and then phylogenetically analyzed. RESULTS: Based on complete coding sequence of HN gene analysis, studied isolates showed close relationship with genotype XIII and subgenotype XIIIa NDV strains. Analysis of both complete HN gene and partial F gene lead to identical results and same classification of studied viruses. CONCLUSIONS: Results of present study are useful for a better understanding of molecular epidemiology of indigenous NDV strains and determining important molecular differences between field and commonly used vaccinal strains related to main immunogenic proteins.

BACKGROUND: The growth and differentiation of skeletal pectoral limb girdle, wing and the ossification centers in these regions after hatching were investigated in pigeons. OBJECTIVES: The aim of this study was to determine the age of physical maturity and radiological and histological assessment of the ossification centers of pectoral limb in quail. METHODS: Fourteen pigeons after hatching were reared in similar and standard conditions and sampled once every 7 to 90 days. RESULTS: According to radiological and histological results, scapula, clavicle, and coracoid were observed after 7th and sternum at 14th day in all specimens. Bones of the humerus, hadius, hlna, hetacarpus II, hetacarpus III, hetacarpus IV, and proximal phalanx of digit III were observed after the 7th day and radial carpal bone, ulnar carpal bone, proximal phalanx of digit II, distal phalanx of digit II, and distal phalanx of digit III at the 14th day and head of humerus, ventral tubercle of humerus, phalanx of digit IV, and dorsal tubercle of humerus at 21st and ventral condyle of humerus and dorsal condyle of humerus at 28th. The histological results were evaluated based on prepared tissue sample from the proximal humeral portion. Lack of bone marrow was observed in all 1st day`s tissue samples and bone marrow conformation was commenced after the 7th day. The growth plate was not observed in all the samples and this issue is complementary to the information obtained from radiographic examination. CONCLUSIONS: According to this study, time taken to complete the ossification process and the formation of all parts of pectoral limb girdle and wing is 28 days after hatching.

BACKGROUND: Femoral head osteotomy in cases of fractures or degenerative diseases is as a routine surgical procedure. Less duration of the healing period and the creation of minimal fibrous tissue and its replacement with bone tissue can be effective in return to health. OBJECTIVES: The aim of this study was to evaluate the histopathological changes following administration of platelet-rich plasma at the site of removed femoral head in rabbits. METHODS: Twenty male New Zealand white rabbits were distributed into two groups including: control (underwent femoral head osteotomy, FHO) and treatment (underwent FHO and planting the platelet-rich plasma on surgical site FHO₊ PRP). In both groups after general anesthesia, femoral head was removed using standard method. In group 2, pre-prepared auto log PRP was used at the site of surgery. After eight weeks all animals were euthanised, femur and its surrounding healing tissues were cut 2cm far from the head of femur and removed. Slides were prepared from each sample through serial sectioning and were stained with H&E and Mason Trichrome. Qualitative changes such as granulation tissues, cartilage and bone formation and their organization and timeliness, thickness of collagen fibers and cellular changes were compared. To quantify the changes, whole surface of the Mason Trichrom stained samples underwent scan with ×50 magnifications and then area of different new formation tissues was measured. Average occupancy levels of each tissue, their ratio to whole surface of sample and to each other in two groups were calculated and compared. RESULTS: In PRP treated group ratio of cartilage tissue to granulation tissue and formation of bone to granulation tissue is significantly more than these parameters in control group. Results indicate faster healing on the damaged area in group 2. Also in group 2, cartilage and bone tissues formation in the healing process was more orderly. CONCLUSIONS: PRP could accelerate healing of bone tissue that is cut at the head of the rabbit’ s femur. So it seems that the use of PRP as a treatment protocol in these cases could be suggested.

BACKGROUND: Mineral elements for various activities such as biochemical, productive and reproductive functions of animals are necessary and their concentration in the body of animals, under the influence of soil and plants will be different in each region. OBJECTIVES: The aim of present experiment was determination of concentration of macro and micro minerals in blood of gazing camel in the pastures of Khuzestan. METHODS: Eighty-eight camels under 11 classes, according to age and physiological situation in Hoveyzeh, Jofeir and Abadan-Khoramshahr regions were investigated during the autumn and winter grazing seasons. RESULTS: Concentrations of calcium, magnesium, sodium, potassium, chloride and iron in serum of all class of camels were in the optimal range, compared to the critical level. The concentrations of camels blood Zn and Cu were under critical level, so they were deficient, the P was near the deficiency threshold. Concentration of Mn was low. CONCLUSIONS: Therefore, as important roles of minerals in improvement of production and reproductive situations of camels in these regions, the mineral status of feedlot must be improved by proper mineral supplementation, the results of present experiment could be useful.