Feyz Medical Sciences Journal
Year:2017 | Volume:21 | Issue:3|Papers Count:14

Background: Candidiasis is a prevalent disease which is caused by different species of Candida. Herbal drugs (e.g. ginseng) were traditionally administrated for the treatment of different diseases. This study was carried out to compare the effect of alcoholic extract of ginseng with Itraconazole againstCandida albicans (C. albicans) and Candida krusei (C. krusei).Material and Methods: This cross-sectional study was crried out on 22 and 8 species of C.albicansand 8 C.krusei, respectively which were isolated from vagina, urine and sputum of the patients. Using the CLSI M27 and disk diffusion methods the susceptibility test was done by Itraconazole (10 mg) and ginseng extract (1, 2, 4, 8, 16, 32, 64 and 128 mg.ml-1). The standard species of C. albicans (PTCC 5027) and C. Krusei (PTCC 5295) were used for the quality control purposes.Results: The lowest and highest minimum inhibitory concentration (MIC) for C. albicans and C. Kruzei was 0.0625 and 0.5 mg.ml-1, respectively for Itraconazole using the microdilution method. However, the lowest MIC and minimum fungal concentration (MFC) for alcoholic extract was 64 mg.ml-1.The highest inhibition zone for C. albicans was 14 and 14-32 mm for alcoholic extract and Foritraconazole, respectively. Using the two methods no significant difference was seen between the alcoholic extract of ginseng (64 and 128 mg.ml-1) and the drug. (P<0.05).Conclusion: Considering the MICs and disk diffusion results, the ginseng extract (64, 128 mg.ml-1) shows considerable antifungal effects compared to Itraconazole.

Background: Hyoscine-N-butylbromide is a potent anticholinergic agent with its prominent spasmolytic action on uterus and cervix smooth-muscle in the first stage of labor. This study aimed to evaluate the effect of Hyoscine-N-butylbromide on the acceleration of labor and rupture of the cervix in primigravida women.Materials and Methods: This double-blind randomized clinical trial was conducted at Ali Ibn-e- Abi Talib hospital of Zahedan on primigravida women (n=105). With the initiation of active phase of labor either Hyoscine-N-butylbromide (40 mg, i.v.) or Saline (2 ml) was administered. Duration of the first, second and third stages of labor and the rate of cervix rupture were compared in two groups.Results: A significant difference was seen for the duration of the first stage of labor (181±59.1 versus 208.2±48.5 min) in two groups (P=0.012).There was no significant difference for the duration of the second and third stages of labor. Although rate of cervix rupture was lower in the Hyoscine group, no significant difference was observed between the two groups.Conclusion: Hyoscine-N-butylbromide is effective in accelerating the first stage of labor and has no effect on the rate of cervix rupture and postpartum hemorrhage.

Background: Although some studies have reported the health-related benefits for the pomegranate seed oil (PSO), there is not enough information on its combined effect with exercise. Therefore, in this study the effect of supplementation with pomegranate seed oil followed by resistance exercise on insulin resistance and lipid profile was considered in nonathletes men.Materials and Methods: In this semi-experimental double-blind randomized study nonathletic male (n=14) were divided into two groups: Exercise+Supplementation (n=7) and Exercise+Placebo (n=7). Both groups performed resistance training for 4 weeks (3 sessions per week). The experimental group consumed 2 capsules of pomegranate seed oil (400 mg) and the control group received 2 placebo capsules daily. Glucose, fasting insulin, total cholesterol, triglycerides, LDL-C, HDL-C, were measured at the beginning and end of the study. Insulin resistance was estimated using homeostasis formula (HOMA-IR).Results: While the average concentration of HDL-C in Supplement+Exercise group was significantly increased compared to pre-test, no significant increase was seen compared to Placebo+Exercise group (P<0.05). Between and within group comparison for the changes in total cholesterol, triglycerides, LDL-C, glucose, fasting insulin and insulin resistance was not significant.Conclusion: According to the results, it can be concluded that 4 weeks of resistance training followed by PSO supplementation, except for HDL-C, has no significant effect on the other lipid profiles and insulin resistance in healthy non-athlete men.

Background: Extended-spectrum beta-lactamases (ESBL) producing isolates of Klebsiella pneumoniaewhich have been increased in the hospitals were resulting in limitation of therapeutic options. The aims of this study were to evaluate the antibiotic susceptibility pattern and presence of ESBL genes in clinical isolates ofKlebsiella pneumoniae from patients admitted to the intensive care units (ICUs).Materials and Methods: In this descriptive cross-sectional study, a total of 65 Klebsiella pneumoniaestrains were isolated from ICUs of educational hospitals in Tehran. Identification was performed using biochemical tests and the antimicrobial susceptibility was performed as recommended by the Clinical and Laboratory Standards Institute (CLSI). Molecular analysis of the ESBL genes was performed by Multiplex PCR (M-PCR).Results: Most of the isolates were resistant to Cotrimoxazole (72.3%), Gentamicin (67.7%) and Ampicillin (69.2%) and the highest susceptibility was seen for Ciprofloxacin (50.8%) Tetracycline (49.2%), Imipenem (46.3%) and Ceftriaxone (43.1%). Among the ESBLproducing genes, bla CTX-M (55.3 %) was the most prevalent, followed by bla TEM (41.5 %) andbla SHV (10.7 %). The results showed that 1.5 % of the isolates had concurrently blaTEM/ bla SHV and bla SHV/ bla CTX-M genes and 21.6% of isolates the bla TEM/ blaCTX-M genes.Conclusion: These findings reveal the high prevalence of multi drug resistant and ESBLproducing Klebsiella pneumoniaein patients hospitalized in ICUs and emphasize the need for appropriate infection control policies.

Background: Bacterial cellulose membrane (BCM) produced by Gluconacetobacter xylinus is an advantageous bacterial product and because of its unique properties could be used as an ideal dressing. The aim of this study was to consider the capability of this biomaterial in the release of Metronidazole. In the case of proving this capability, it provides the basis for the production of a dressing containing this type of antibiotic.Materials and Methods: In this study, BCM was initially synthesized by Gluconacetobacter xylinus. The BCM was loaded by Metronidazole. Then the release process was considered in distilled water and buffer phosphate Saline. The ultra violet spectrophotometry was applied for measuring the concentration of the released drug.Results: The chemical structure of bacterial cellulose was confirmed by Fourier Transform Infrared (FT-IR) spectroscopy. The release of Metronidazole in distilled water and phosphate buffered Saline was reached to 84.27% and 84.71%, respectively. Due to higher release in phosphate buffered Saline media, it seems that the trend of release in vitro provides efficient results.Conclusion: Results of this study provides the basis for future research on supplying an ideal dressing from this microbial product.

Background: The aim of this study was to investigate the response of glycemic index and liver tissue damage to aerobic training along with coriander seed extract in Streptozotocininduced diabetic rats.Materials and Methods: diabetic male Wistar rats (n=40, average age: 12 weeks; weight: 130±10 g) were randomly divided into four groups: Control, Extract, Exercise and Exercise+Extract. The diabetes model in rats was induced with a single injection of Streptozotocin- (60 mg/kg) dissolved in citrate buffer (0.05 mole). The high glucose level of 250 mg/dl is defined as the diabetic criterion. The extract was administered orally (150 mg/kg/day). The exercise program was six weeks of aerobic exercise, 5 times a week with 50-55% of maximal oxygen consumption.Results: The application of aerobic training followed by coriander seed extract in diabetic rats had a significant effect on total serum glucose (P=0.002), AST (P=0.001), ALT (P=0.005) and ALP (P=0.033); however, it had no significant effect on insulin level (P=0.656) and insulin-resistance (P=0.458).Conclusion: It seems that the combination of a regular aerobic exercise and coriander seed extract in diabetic rats had beneficial effects on liver tissue damage and possibly can prevent and improve liver tissue damage via the reduction of the of some liver tissue damage markers.

Background: Obesity and its related disorders are the cause of cardiovascular disease and an increased risk of morbidity and mortality. Nevertheless, the results of studies indicated that exercise training have beneficial health effects on obesity and risk markers of cardiovascular disease. Therefore, the purpose of this study was to examine the effect of 8 weeks of resistance training on lipocalin2 (LCN2) and fatty acid binding protein (FABP) in obese adult males.Materials and Methods: In this quasi-experimental study, the obese adult men (n=24) were randomly assigned into Control and Exercise groups. Exercise group was participated in a resistance exercise program with 50-80% of one Repeated- Maximum (IRM) intensity for 8 weeks (three times per week). Venous blood samples were collected before and after the exercise. Moreover, body composition indexes were measured at pre- and post-training programme.Results: Resistance exercise caused a significant decrease in the body fat percent, waist circumference and waist to hip ratio (WHR) in Exercise group (P<0.05). Moreover, the results indicated a significant decrease in Exercise group for LCN2 concentration after resistance training (P=0.002) compared to Control group (P=0.799). Also a significant decrease in FABP4 concentration was found in Exercise group (P=0.002).Conclusion: The results of the present study suggest that 8 weeks of resistance exercise improve body composition and decrease the new cardiovascular biomarkers in obese men. However, further research is necessary to understand the molecular mechanisms involved.

Background: Expressed emotion is one of the key factors resulting in relapse of psychiatric disorders. Expressed emotion is a critical, hostile and emotionally over-involved attitude that relatives have toward a family member with such disorder. Since stress from the expressed emotion leads to relapse in person with a disorder, the present study aimed to investigate the effect of mental training based on the acceptance and commitment on the regulation of expressed emotion in families of patients with bipolar disorder.Materials and Methods: This quasi-experimental study was carried out on families of patients with bipolar disorder referring to Kargarnejad hospital (Kashan, Iran). The purposive sampling method was used to select the participants. As an intervention acceptance and commitment-based psychoeducation of the families was held in 10 sessions. Expressed emotion questionnaire was completed by relatives pre- and post-intervention.Results: The results showed that the level of expressed emotion reduced after the intervention in families of patients with bipolar disorder (P<0.001). A significant reduction was observed in the level of expressed emotion in all four subscales (negative attitudes, tolerance/expectations, harassment and intervention and emotional response) (P<0.001).Conclusion: Family psychoeducation based on the acceptance and commitment can be effective in the regulation of expressed emotion in families of patients suffering from bipolar disorder.

Background: The ageing of population is an increasing phenomenon worldwide. Cardiovascular diseases are one of the most important chronic disease in ageing. The aim of this study was to investigate the correlation between the anthropometric indexes and risk factors of cardiovascular diseases among the elderly population in Amirkola (Mazandaran, Iran).Materials and Methods: This descriptive-analytical cross-sectional study was a part of the Amirkola Health and Ageing Cohort Project (AHAP). Demographic information was collected using a standard questionnaire. Fasting blood samples were collected from all participants to measure the serum level of lipids. Measurement of systolic and diastolic blood pressure and anthropometric indexes [e.g. body mass index (BMI), waist circumference (WC), waist to hip ratio (WHR) and waist to height ratio (WHR)] were done for all participants.Results: One thousand five hundred and six elderly people of Amirkola (age>60 years) were participated in this study. There was a positive and significant correlation between the BMI and systolic and diastolic blood pressures, total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C) and triglyceride. The WC had a positive and significant correlation with systolic and diastolic blood pressures, HDL-C and triglyceride. The correlation between WHR and systolic and diastolic blood pressures, HDL-C and triglyceride was positive and significant; however the correlation between the anthropometric indexes and TC and low density lipoprotein-cholesterol (LDL-C) was negative and significant. The WHR had a significant positive correlation with all studied risk factors.Conclusion: According to the findings of present study, WHR index had stronger correlation with risk factors for cardiovascular disease in the elderly.

Background: Because of its role in the incidence of serious microbial infections, the contamination of dental unit water lines is concerned. The aim of this study was to evaluate the bacterial contamination of dental unit water lines to gram-positive cocci at Kerman.Materials and Methods: This cross-sectional study was carried out in Environmental Health Engineering Research Center of Kerman University of Medical Sciences (KUMS) during 2015. Randomly 4 units among each of the departments of Dental School of KUMS (Pediatrics, Orthodontics, Oral surgery, Maxillofacial and Oral medicine) were selected. Samples were transported to the laboratory in sterile containers.Results: No contamination to gram-positive cocci in drinking water samples were reported. Among the water samples taken from Dental School the highest bacterial count was for the Oral medicine department (the cup filler unit 26.25 cfu/mL and 32.18 cfu/mL and the lowest bacterial count was for Oral surgery, Maxillofacial departments [parts exposed to the weather unit 23.25 (cfu/mL) and 24/43 (cfu/mL)].Conclusion: The prevalence of gram positive cocci in dental units of the departments was high. The dentists should care to the presence of microorganisms and take preventive measures to reduce the risk of infection among the department staff and patients.

Background: With the progression of aging process, the degenerative process affect all organs throughout the body, including the intervertebral discs. The degeneration of intervertebral discs is the most important etiology for the back pain in human. Hence, having the knowledge of alterations in biomechanics of this structure due to the degeneration is of special importance.Materials and Methods: In this cross sectional study all patients referred to the Spinal Cord Clinic of Bahonar hospital (Kerman, Iran) because of their back pain were participated. The upright anteroposterior and lateral flexion-extension radiographs and MRI of the spine have been ordered for the diagnosis and treatment of the patients with back pain. The sagital translation and rotation and also anterior and posterior disc height in different grades of degenerations was analyzed.Results: The severity of the intervertebral disc degeneration has direct relation to aging. Anterior and posterior disc height was decreased with the progression of the degeneration. Translation and rotation in the sagital plane were decreased along with the progression of the grade of the degeneration and in direct relation to the posterior disc height.Conclusion: The degeneration trend is progressing with the advancement of aging and the disc space is decreasing along with the severity of degeneration, followed by a reduction in the translational and rotational movements of the spinal segments, so the grade 3 degeneration has been assumed to be equivalent to the stabilization phase of the motion segment.

Background: Neonatal hyperbilirubinemia is a common and preventable cause of sensoryneural hearing impairment, which can cause difficulties in the development of speech and communication. This study was conducted to detect the toxic effect of hyperbilirubinemia on the brain stem and auditory tract in neonates with icterus admitted to Shahid Beheshti Hospital in Kashan, Iran.Materials and Methods: This cross-sectional study was conducted on 98 neonates with increased indirect bilirubin admitted to Shahid Beheshti Hospital in Kashan during 2014-2015. The patients were referred to Matini Hospital for the assessment of the auditory brainstem response (ABR); wave latency and interpeak intervals of the waves were also evaluated. According to the serum bilirubin level, the neonates were allocated into two groups; one group had a serum bilirubin level of 13-20 mg/d and another group had a bilirubin level more than 20 mg/d.Results: From 98 neonates, 26 (26.5%) had a bilirubin level more than 20 mg/d and 72 (73.5%) had a bilirubin level of 13-20 mg/d. Also, 46.1% of the neonates in the first group (bilirubin>20 mg/d) and 2.8% of the neonates in the second group (bilirubin 13-20 mg/d) had abnormal ABR (P<0.0001). There was a significant difference between the mean latency time of III and V waves and the interpeak intervals of I-III, I-V, and III-V waves in neonates of the two groups (P<0.05).Conclusion: An increased indirect bilirubin level (>20 mg/d) can cause an auditory processing disorder in neonates. So, performing ABR for screening and early detection of bilirubin toxicity can be recommended as a necessary audiologic intervention in all cases of severe neonatal hyperbilirubinemia.