Drought stress is one of the most important factors in reduction of crops production. An experiment was conducted on 7 cultivars of wheat in randomized complete-block and split plot design with three replications in order to detect the tolerant cultivars against drought stress also Assessment the physiological and biochemical processes associated with drought tolerance to optimize the supplementary irrigation time. The mentioned experiments were performed under supplementary irrigation conditions at the stemming, boating and flowering steps also under non-irrigated condition. The experiments were done in two consecutive years (1394 and 1395) at Dehgolan field, depending on Faculty of Agriculture and Natural Resources, Kurdistan University. In the present study, peroxide-malondialdehyde level (MDA), total protein content, and the activity of catalase, ascorbate peroxidase, guaiacol peroxidase and superoxide dismutase were measured. Based on the results, a significant interaction was measured, in the most of attributes, between cultivar and supplementary irrigation. Compared to the control sample, Supplementary irrigation leads to decrease H2O2' content. The maximum values of H2O2 and MDA were measured for Aras, Abu Ghraib and Tamuz cultivars, respectivelywhile the minimum values of them were obtained for Azar-2. Curiously, supplementary irrigation caused to increase the activity of catalase, and superoxide dismutase for all cultivars also total protein content (except Aras cultivar) and ascorbate peroxidase (just for Axad 65, Rezgari, Smito and Azar-2 cultivars). According to the results, Axad 65, Rezgari, and Azar-2 cultivars in flowering stage could control exist conditions, with less energy consumption, through regulation and optimization their defense mechanisms.