Aims: One of the strategies for coping with oxidative stress and, subsequently, prevention complication of aging is the use of antioxidant supplements and exercise training. Therefore the aim of this study was to evaluate concurrent training and supplementation of L-arginine on stress oxidative and lipid profile in elderly men. Materials & Methods: In this quasi experimental study, 44 older volunteering men (mean age: 67. 77± 4. 61), were selected by simple convenience sampling from Isfahan city in 2017 and by using NCSS PASS 11 software, they were randomly divided into four equal-sized groups (each n=11): Concurrent Training +Supplement (ES) group; Concurrent Training +Placebo (EP) group; Supplement (S) group and Control (C) group. The ES and EP groups performed the exercise protocol with eight weeks of concurrent training, three sessions per week. Group ES and group S consumed 1000 mg of L-Arginine every morning and one hour before the exercise. Maltodextrin were used as placebo. Biochemical samples were assayed by ELISA method and multivariate longitudinal model were used for analysis of data in the level of significance p<0. 05. Findings: Results showed that, joint effect of training and supplementation was more than the effect of the exercise or supplement alone on variable of stress oxidative 8-OhdG (β =-5. 16, p<0. 001), TAC (β =-2. 30, p<0. 001), and MDA (β =3. 50, p<0. 001) and variables lipid profile LDL (β =-20. 82, p<0. 001), VLDL (β =-4. 73, p<0. 001), HDL (β =-3. 81, p<0. 022), TC (β =-21. 15, p<0. 001), TG (β =-24. 74, p<0. 001). Conclusion: Findings of this study suggest that the simultaneous use of concurrent training and supplementation of L-Arginine improves the system antioxidant and lipid profile in the elderly men.