Dietary phosphorus requirement of narrow-clawed crayfish, Astacus leptodactylus, was determined using five pelleted diets containing equal levels of energy, protein and lipid. Five experimental diets were prepared using a basal diet containing casein, gelatin, dextrin, starch, fish oil, soya oil, vitamin mixture, and mineral mixture tree of phosphorus. Various concentrations of phosphorus (containing of 0, 0.5, 1, 1.5, 2 percentages) provided through adding sodium monodihydrogen phosphate in the basal diet. Crayfish with initial mean weight of 8.16±1.91g was fed experimental diets for 80 days. The diet with 0% phosphorus supplementation yielded the lowest weight gain (1.06g) and the highest weight gain of 2.34g and 2.52g were achieved in the diets containing 1.5% and 2% phosphorus, respectively. There was significant difference between the dietary levels of phosphorus and weight gain (p<0.05). Moreover, the diets with low phosphorus supplementation levels (0-1%) showed the poorer survival rate. Although the lowest survival rate was observed at the 1% phosphorus level and the highest at 1.5% and 2% phosphorus levels, no significant difference (p>0.05) between survival rates in the trial diets was found.Optimal phosphorus requirement in the diet of narrow-clawed crayfish was estimated with broken line regression analysis to be about 1.3%. From these findings, it may be concluded that a dietary phosphorus supplement is necessary for narrow-clawed crayfish. So, it is recommended to use the dietary hosphorus concentration of 1%-1.5% in applied diets formulation.