Armaghane Danesh
Year:2015 | Volume:20 | Issue:3 (98)|Papers Count:11

Background & aim: Utilizing tissue engineering techniques and designing similar structures of the damaged tissues require the use of tools such as scaffolds, cells, and bioactive molecules in vitro. Meanwhile, appropriate cell cultures with the ability to divide and differentiate on the natural scaffolds lacking features like immunogenicity and tumorgenesis is particularly important. Adipose tissue has attracted researchers’ attention due to its abundance of mesenchymal stem cells and its availability through a liposuction. The purpose of the present study was to investigate the reproducibility and viability of the adipose-derived stem cells on natural scaffolds of fibrin glue and agarose.Methods: In the present experimental study, the isolation and identification of the mesenchymal stem cells was performed on tissue obtained from liposuction. The tissues were extensively washed with PBS and were digested with collagenase I, then the mesenchymal stem cells were isolated. The cells were cultured in RPMI medium supplemented with antibiotic. Subsequently, the expression of cell surface markers including CD34, CD44, CD90, and CD105 were analyzed by flow cytometry to confirm the mesenchymal cells. After preparing fibrin glue and agarose scaffolds, the viability and proliferation of the adipose tissue-derived mesenchymal stem cells were examined at the period of 24, 48, and 72 hours by MTT and ELISA assays. The obtained results were analyzed by SPSS ver.19.Results: The results of adipose tissue-derived mesenchymal stem cells culture on the fibrin glue and agarose scaffolds indicated that cell viability on fibrin glue and agarose scaffold were 68.22% and 89.75% in 24 hrs, 64.04% and 66.97% in 48 hours, 222.87% and 1089.68% in 72 hours respectively. Significant proliferation and viability cells on a synthesized agarose scaffold were seen compared to the fibrin glue scaffold after 72 hrs. The viability of the cells significantly increased on the agarose scaffold.Conclusion: Due to stability and permeability of scaffolding agarose, it seems that scaffolding agarose created better adhesion of cells in the performance of cell proliferation process compared with fibrin glue scaffold.

Background & aim: Gastric cancer is one of the most common malignancies and its early diagnosis can be effective in their treatment. Loss of genomic stability and the resulting gene alterations appears to be a crucial molecular and pathogenic step that occurs early in the gastric carcinogenesis process. X-ray repair crosscomplementing gene 1 (XRCC1) is one of the most important DNA repair genes. The XRCC1 protein plays an essential role in base excision repair. Coding polymorphisms of the XRCC1 gene have been shown to affect the DNA repair capacity and to be associated with genetic susceptibility to carcinogenesis. The aim of this study was to investigate the association of XRCC1 C26304T gene polymorphism with the risk of gastric cancer.Methods: In the present case-control study, genomic DNA was extracted from 110 cases with gastric cancer and 116 normal subjects as control group. Two groups had similar age, sex and ethnic background. The Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for detection the genotype and allele frequencies of XRCC1 single nucleotide polymorphism in each subject. Data were analyzed using the MedCalc (V.12.1) software and Chi-square test and P<0.05 was considered significant.Results: C and T allele frequency in patients 0.88, 0.11 and in healthy subjects were 0.94, 0.06 respectively (p>0.05). The C and T allele frequencies in cases were 0.88 and 0.11, respectively and 0.94 and 0.06 in healthy individuals (p>0.05). The distribution of CC, CT and TT genotypes among cancer cases were 78.2%, 20%, 1.8%, and In the control group were 88.8%, 10.3%, and 0.9% respectively. The genotype frequencies were significantly different in the cases and controls. The CT genotype was significantly associated with an increased risk of gastric cancer (p=0.042). In addition, the distribution of the CT+TT genotype was different between case and control subjects (p=0.033).Conclusions: Results revealed that the Screening of XRCC1 gene polymorphism could be a marker in personal sensitivity to gastric cancer and useful in cancer treatment and prevention process. Confirmation of this finding needs to be repeated with similar studies in larger population.

Background & aim: Major depression disorder is one of the common mental disorders that the way of cognitive emotional regulation influences in development or reducing symptoms of this disorder. Since cognitive emotional regulation plays an important role in coping with life problems, this disorder causes emotional disturbances such as major depression. The aim of this study was to compare the efficacy of cognitive behavior therapy and mindfulness-based therapy to improve cognitive emotion regulation in Major depressive disorder Methods: the present experimental study was conducted using a pretest- posttest approach with two groups.30 participants with major depression disorder in Sfarayen counseling and higher education centers were randomly selected and divided in two groups of 15. Each group was under cognitive-behavior and based on mindfulness treatment during 8 sessions, each 90 minutes. They were analyzed using clinical diagnostic interview and cognitive emotional regulation questionnaire.Independent t-test was used for data analysis.Results: t-test results and comparison of differences between pretest and posttest scores indicated that mindfulness-based therapy in improving cognitive emotion regulation in patients with major depression was more effective than cognitive-behavioral therapy (t=-2.85, p=0.008).Conclusion: The cognitive behavioral and based on mindfulness treatment were effective in cognitive emotional regulation improvement, since treatment of based on mindfulness puts emphasis on current feelings and avoidance of self-judgment, and can also be more effective than cognitive behavioral method in using adaptive strategies of cognitive emotional regulation by patients with major depression.

Background & aim: Breast cancer is the most common cancer among women and one of the factors threatening the health of women worldwide. Leptin is a 16 kD glycoprotein hormone produced predominantly by white adipose tissue. Leptin binds to receptors in the hypothalamus and plays a key role in regulation of metabolism. Both leptin and leptin receptor have recently been implicated in processes and progress leading to breast cancer initiation. The aim of this study was to identify if there is association between leptin and pathological indexes in patients with breast cancer Methods: 45women with breast cancer were enrolled. Serum leptin levels of patients were measured by the ELISA method. Pathological information such as stage of the breast cancer, Hormonal receptor (ER, PR) and Her2 status in these patients were determined.Result: Results revealed that the patients who were in stage one and two, the mean serum leptin level was (34.18±21.22 ng/ml) And patients who were in stage three and four, the mean serum leptin level was (32.21±21/93 ng/ml). Also the mean serum leptin levels in patients whose receptor status of ER, PR and HER2 positive were (35.90±23.55, 35.74±23.91and 37.02±24.25ng/ml), respectively. The Patients whose receptor status of ER, PR and HER2 negative were 26.64±13.13, 28.17±14.26and31.32±19.9ng/ml respectively. No significant association was found between leptin leveland stage of the breast cancer, hormonal receptor (ER, PR) and Her2 status in Patients with Breast cancer (p>0.05).Conclusions: In this study, no association was found between serum leptin level and pathological indices in women with Breast cancer in Yasuj, Iran.

Background & aim: One of the most common clinical problems among individuals is thyroid nodule diseases which are characterized by one or more nodules in the thyroid and are usually benign. It can be said that thyroid cancer is the most common endocrine cancer worldwide. This study aimed to determine the risk factors for cancer in patients with thyroid nodule in Mazandaran province, Iran, using parametric survival analysis.Methods: In the present historical cohort study, 26, 730 patients with thyroid nodules who were referred to health care centers from July 2002 to March 2008 were identified. Parametric log-normal and log-logistic models were compared with and without taking frailty into account. The criterion for comparing models was Akaike's criterion. All calculations were performed with the SPSS software and the significance level was considered 0.05.Results: The mean time of the conversion of thyroid nodules to cancer in patients was found to be 29.32 months. Using Kaplan-Meier method, survival rates of one year, five years and ten years of nodule conversion to cancer was calculated 94.6, 88.6 and respectively. According to the log rank test age (p=0.03), hypothyroidism (p=0.01), bilateral nodules (p<0.001), a multi-nodular goiter (p<0.001), TSH hormone (p<0.001), T4 hormones (p=0.005), cholesterol (p=0.03), creatinin levels (p=0.001) a significant relationship was seen. Based on the Akaike's criterion, the lognormal model which takes frailty into account best fits to the data.Conclusion: Based on the log-normal model with frailty, It can be concluded that the thyroid nodule patients with abnormal TSH hormone are 6.55 times more likely to develop risk of thyroid cancer than patients who had normal TSH hormone overall. This model also indicated that patients who had heart palpitations are 5.52 times more likely to develop risk of cancer than patients who did not have heart palpitations.

Background & aim: learning style (s) is one of the factors effective learning. Information about students learning styles can aid the presentation of teaching appropriate to their individual style. The purpose of this study was to determine the learning styles of Yasuj nursing and midwifery students based on the VARK model in 2013.Methods: The present research was a descriptive- analytical study which was conducted on 140 students from nursing and midwifery faculty of Yasuj university of Medical Sciences in the academic year of 2013. Sampling was done using census method, and data were collected using demographic information questionnaire followed by questionnaire of learning styles. The data were analyzed using SPSS software version 18, and descriptive- inferential statistics.Results: The mean age of the students was 20.68±1.34. Of the total of 140 students, 98 (70%) just selected only one type of the learning styles, whereas, 42 students preferred combination of different styles. Readingwriting style was the prevailing style among single learning style which selected by 43 students but the most frequent style among multi style was double style which was selected only by 15% of the participants.Conclusion: Most of the students had an individual learning style. Identifying the dominant learning style (s) of the students and adjusting them to the teaching methods of lecturers and also developing lesson plans based on learning styles could improve educational objectives.

Background & aim: A developmental delay in children is considered as one of the world's health problems in developing and developed countries. This has a massive impact on children’s health and eventually has broader scale effect on society. The purpose of this study was to assess the developmental status of children age 4-24 months to teenage mothers referred to health care centers related to Yasuj University of Medical Sciences, Yasuj, Iran, in 2013.Methods: The present cross-sectional study was conducted on 200 mothers less than 19 years of age with children of 4-24 months referred to health centers of Yasuj University of Medical Sciences which were selected by multistage sampling. Data collection was conducted using demographic, midwifery ages and stages questionnaire in children aged 24-4 months. The data were analyzed using descriptive statistics, t-test and chi-square test.Results: The results showed that the mean age of mothers during pregnancy were16.64±1.22 years with age range 12 to 18 years. Prevalence of delayed development in 4-24 month children was reported to be 7%. Most developmental delay was observed in gross motor area and the lowest was in the private personal and social domains. In this study, a significant difference was seen in birth weight between two groups of children, so that birth weight of children with developmental delay significantly lowers than children with normal development (P=0.027).Conclusion: the Pregnancy under the age of 19 years is considered as risk factors for children’s developmental disorder that by the implementation of appropriate education to reduce high-risk pregnancies in this age range can be prevented the developmental disorders and their adverse outcome.

Background & aim: Drug misuse is defined as inappropriate administration or usage of drug which can be preventable. The aim of study was to identify the consistency rate of drug record information between cardex and patients medical cards in teaching hospitals of Yasuj, Iran Methods: The present descriptive sectional study was conducted in teaching hospitals of Yasuj in 2012. Case Sampling was convenience sampling. A number of 400 patient’s charts were selected and drug orders were compared with cardex and drug patient’s cards. Statistical analysis was performed by SPSS software and descriptive tests.Results: Of 400 charts, only 1670 charts had drug orders and there were a total 337 of drug errors in cardex and drug cards. The highest rate of inconsistency of drug details was related to entering drug routes into cradex and drug cards that were reported 41% and 43.2 % respectively. In addition about 5% and 23.4% of administrated drugs were not recorded in cardex and drug cards. It is also indicated that 1% and 2.5 % of deleted drug orders in the chart were not deleted in cardex and drug cards.Conclusions: There are relatively large numbers of inconsistency cases of drug details between cardex, drug cards and patient’s charts. It is necessary to clarify the issue, and also performed new methods of drug registration in the hospitals.