In order to determine genetic relationship of 64 genotypes of Durum wheat (including 6 indigenous and 58 exogenous genotypes) experiment was conducted in the form of Simple Lattice Design during 2002 - 2003. In this study the traits of consideration was as follow: seeding time, heading time, flag leaf area, plant height peduncle length, number of nodes. spike length, internodes length, number of fertile tillers, biomass, number of kernel in spike, 1000 kernel weight, grain yield and harvest index. Results archived from Combined analysis showed a significantly difference (P<0.01) between above mentioned traits which explains genetic variety between genotypes. In order to determine genetic relation between lines, Cluster analysis in Ward method was performed and genotypes in question were divided into 5 groups. Native genotypes in an individual group and all other exogenous genotypes (depending on their genetic similarity and/or differences) in 4 other groups which shows similarity between native lines and also difference between exogenous ones. In some groups, several traits showed desirable traits value more than mean value which could be useful to enhance the value of such traits during hybridization.