One of the main area for cultivation of garlic in Iran is Hamedan province. This plant has the best growth condition in places with moderate climate. The aim of this study was identification of garlic fungal disease agents in Hamedan province. In order to identify these agents, during years 2006-2008 more than 693 samples suspected to fungal disease were collected from different places and were transferred to Laboratory. Pieces from edge of diseased part of garlic were cut and cultured on media including PDA, PCA, CLA, G 25%N, SNA, MEA, CYA, HCA and BNPRAH.Totally 648 fungal isolates wee obtained. According to colony character and microscopic features of conidium, conidiophore, sc1orot and features of sexual organs the following species were identified: Aspergillus niger, Penicillium aUn, Fusarium oxysporum, F.proliferatum. F. so/ani, Embellisia allii, Pythium graminicola, Rhizoctonia solani, Puccinia allii, Ulocladium allii, Botrsis aUii, Cladosporium alii.Genera Rhizoctonia, Ulocladium, Pythium and Botrytis, are reported for I first time in garlic in Hamedan provimce. Pathogenicity of identified species on garlic were achived and all of them were pathogenic on garlic.