Vesicular Arbuscular mycorrhizae fungi created a symbiosis with the root of agricultural plants, with improvement in absorbing some of the food elements will be a cause increase in plant growth. In this research, one species potential of endomycorrhizae fungi separated from a farm's soil were evaluated upon ontogeny process of counter effects on fungi and higher plants. In this scheme, in the first step, the fungi's spore separation and purification have been taken place with wet sieving. In the second step, inoculation and reproduction of fungi will take place with Sorghum vulgare plant helping and pot culture method. In the third step specifications regarding the spore, growth and development of fungi vegetative organs in root tissue, have been studied upon a careful microscopic survey. At fourth steps, the effect of spore concentration, light and nutrition solution concentration, have been studied on both plants maize and sorghum. The results have shown that in reproduction Glomus species, making use of new polluted roots as an inoculating matter is much more effective than spore. Statistical results show that in all three tests the effect of spore concentration, light and concentration of nutrition solution on the rate of colonization, based on t-test exams and analysis of Variance, between treatments there are a statistical meaning differential (P<0.05). About the role of original spore concentration and light, on the rate of inoculation and colonization percentage, the results show that increasing of spore concentration and light will increase rate of symbiosis and also increase of concentration of nutritional solution will decrease percentage of colonization.