Inhibitory Effects of Dutasteride on TLR4: An In vitro Pain Study

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAHERAN LEILA; ZALI HAKIMEH; Sharifi Kazem; YAZDANI MOHSEN; Ajoudanian Mohammad; SAFARI MIR SHAHRAM; RAJAEI SAMIRA; DABBAGH ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY Year:2022 | Volume:21 | Issue:5 Page(s): 574-583

Download Full-Text

View:

Download:

184

Cites:

Information Journal Paper

Title

Inhibitory Effects of Dutasteride on TLR4: An In vitro Pain Study

Author(s)

Keywords

Dutasteride

Neuropathic pain

Toll-like receptor 4

Abstract

Dutasteride was potentially proposed to control chronic pain by Toll-Like Receptor 4 (TLR4) inhibition through its effect on TLR4 expression, Myeloid differentiation primary response 88 (MyD88), Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κ, B), secretory Interleukin-1β,(IL-1β, ), and nitric oxide (NO) in the Lipopolysaccharides (LPS)-stimulated U-87 MG cell line. The human astrocytoma U-87 MG cell line was cultured and incubated with 10 μ, g/mL of LPS for 24 hours to create a neuro-inflammation model, using two different treatment approaches. The first approach included LPS treatment for 24 hours, followed by dutasteride (20 μ, g/mL) incubation for the next 72 hours. In the second treatment approach, the cells were co-incubated with LPS and dutasteride for 72 hours. Expression of TLR4, MyD88, NF-κ, Bp65, and secretory IL-1 was evaluated by Western blotting while expression of NO was assessed by NO assay. TLR4, MyD88, NF-κ, Bp65, and secretory IL-1β,levels increased in LPS-treated cells after 24 hours. Dutasteride significantly decreased the secretion of NO and also, the levels of TLR4, MyD88, and NF-κ, Bp65 in both treatment approaches. No difference in IL-1β,level was seen with the second treatment approach. Dutasteride has anti-inflammatory properties and probably analgesic effects, by mechanisms different from conventional analgesics.

Multimedia

No record.

Cites

The Sun Will Be Shining in the Pain Management Sky

References

No record.

Cite

APA: Copy

TAHERAN, LEILA, ZALI, HAKIMEH, Sharifi, Kazem, YAZDANI, MOHSEN, Ajoudanian, Mohammad, SAFARI, MIR SHAHRAM, RAJAEI, SAMIRA, & DABBAGH, ALI. (2022). Inhibitory Effects of Dutasteride on TLR4: An In vitro Pain Study. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 21(5), 574-583. SID. https://sid.ir/paper/1075485/en

Vancouver: Copy

TAHERAN LEILA, ZALI HAKIMEH, Sharifi Kazem, YAZDANI MOHSEN, Ajoudanian Mohammad, SAFARI MIR SHAHRAM, RAJAEI SAMIRA, DABBAGH ALI. Inhibitory Effects of Dutasteride on TLR4: An In vitro Pain Study. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2022;21(5):574-583. Available from: https://sid.ir/paper/1075485/en

IEEE: Copy

LEILA TAHERAN, HAKIMEH ZALI, Kazem Sharifi, MOHSEN YAZDANI, Mohammad Ajoudanian, MIR SHAHRAM SAFARI, SAMIRA RAJAEI, and ALI DABBAGH, “Inhibitory Effects of Dutasteride on TLR4: An In vitro Pain Study,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 21, no. 5, pp. 574–583, 2022, [Online]. Available: https://sid.ir/paper/1075485/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops