GLYCOSYLATION ENGINEERING OF HUMAN RECOMBINANT PROTEINS IN NEW S2 SYSTEM

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VATANDOOST JAFAR; KHALILI LEILA

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Journal: KNOWLEDGE AND HEALTH Year:2015 | Volume:10 | Issue:3 Page(s): 45-52

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Information Journal Paper

Title

GLYCOSYLATION ENGINEERING OF HUMAN RECOMBINANT PROTEINS IN NEW S2 SYSTEM

Author(s)

VATANDOOST JAFAR | KHALILI LEILA | Issue Writer Certificate

Keywords

DROSOPHILA S2 CELLSQ2

GLYCOZYLATIONQ2

N-ACETYLGLUCOSAMINIDASEQ2

HEXOSAMINIDASEQ2

Abstract

Insect expression systems have been used to achieve high expression of recombinant and complex proteins, but disability of insects in the synthesis of N-Glycan products similar to mammals has been a controversial conflict debate in recent years.Glycosylation products in insects contain high or low end of mannose units. The main reason for this inability is the low level of activity of a number of enzymes including b-N - (1 and 2) acetyl glucosamine transferase I and II, b- (1 and 4) galactosyl transferase, a- (2, 3) and a- (2, 6) sialyl transferase. In addittion, a hexoaminidase that remove N-acetyl glucosamine at the end of glycan products and prevents binding of galactose and Sialic acid to glycan products have been discovered in insects. So the insect cells can be engineered to produce glycan products similar with mammalians and remove blocking agents of synthesis of sialyl and galactose products. In this systematic review, the glycosylation pathways in mammals and insects and engineering of possible glycosylation pathways in S2 cells have been investigated.

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APA: Copy

VATANDOOST, JAFAR, & KHALILI, LEILA. (2015). GLYCOSYLATION ENGINEERING OF HUMAN RECOMBINANT PROTEINS IN NEW S2 SYSTEM. KNOWLEDGE AND HEALTH, 10(3 ), 45-52. SID. https://sid.ir/paper/107806/en

Vancouver: Copy

VATANDOOST JAFAR, KHALILI LEILA. GLYCOSYLATION ENGINEERING OF HUMAN RECOMBINANT PROTEINS IN NEW S2 SYSTEM. KNOWLEDGE AND HEALTH[Internet]. 2015;10(3 ):45-52. Available from: https://sid.ir/paper/107806/en

IEEE: Copy

JAFAR VATANDOOST, and LEILA KHALILI, “GLYCOSYLATION ENGINEERING OF HUMAN RECOMBINANT PROTEINS IN NEW S2 SYSTEM,” KNOWLEDGE AND HEALTH, vol. 10, no. 3 , pp. 45–52, 2015, [Online]. Available: https://sid.ir/paper/107806/en

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