DIAGNOSIS OF ANTI-NUCLEAR ANTIBODY USING CALF THYMUS COATED GLASS IN PATIENT WITH LUPUS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOSSEINI SEYED ZEYNOLABEDIN; MASOOMI KARIMI MASOOMEH; JAFARI SANI MOSLEM; KAZEMI SHAHANDASHTI SADY; LAKOURAJ MANSOUR; GHAFARICHERATI RAMEZAN; TABAR MOLAHASAN AGHIL

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: KNOWLEDGE AND HEALTH Year:2016 | Volume:11 | Issue:3 Page(s): 55-62

Download Full-Text

Persian Verion

View:

1,056

Download:

Cites:

Information Journal Paper

Title

DIAGNOSIS OF ANTI-NUCLEAR ANTIBODY USING CALF THYMUS COATED GLASS IN PATIENT WITH LUPUS

Author(s)

Keywords

SYSTEMIC LUPUS ERYTHEMATOSISQ2

AUTOIMMUNE DISEASEQ2

ANTI-NUCLEAR ANTIBODY TESTQ2

INDIRECT IMMUNOFLUORESCENT ASSAYQ2

THYMUSQ2

Abstract

Introduction: Indirect immunofluorescence test is a most common test for the detection of SYSTEMIC LUPUS ERYTHEMATOSIS (SLE), however, it is very expensive and is not easily available for all field laboratories. We aimed to evaluate the diagnostic performance of five micrometer thick sections prepared from bovine THYMUS for the detection of SLE patients.Methods: Totally 48 serum samples (25 negative and 23 positive) were drained from SLE suspected patients. Bovine THYMUS was purchased from the slaughterhouses. Five micrometer thick sections prepared from bovine THYMUS. Also rat liver was prepared through the laboratory animal dissection, and assayed in parallel with HEp-2 tests. Two fold serial dilutions (1: 40 to 1: 640) were prepared for each serum sample using phosphate buffer solution and assayed for ANA. Immunostained cells were observed using a blue filter equiped fluorescent microscope with 200x magnification and any bright green fluorescence accumulation in the cell nucleus was considered as positive for the presence of ANA.Results: Our findings showed sections prepared from THYMUS cells can be used for the detection of ANA in patient samples and the calculated sensitivity, specificity and accuracy of the test were 82.6%, 100% and 91.7%, respectively. Positive predictive value and negative predictive value of the test were 100% and 86.2%, respectively.Conclusion: We concluded that thymic cells can be easily prepared and used for detection of ANA in SLE patients. However, minor improvements in section preparation and assay conditions as well as determination of a new cut off point is necessary to gain 100% efficiency of the assay.

Cites

No record.

References

No record.

Cite

APA: Copy

HOSSEINI, SEYED ZEYNOLABEDIN, MASOOMI KARIMI, MASOOMEH, JAFARI SANI, MOSLEM, KAZEMI SHAHANDASHTI, SADY, LAKOURAJ, MANSOUR, GHAFARICHERATI, RAMEZAN, & TABAR MOLAHASAN, AGHIL. (2016). DIAGNOSIS OF ANTI-NUCLEAR ANTIBODY USING CALF THYMUS COATED GLASS IN PATIENT WITH LUPUS. KNOWLEDGE AND HEALTH, 11(3 ), 55-62. SID. https://sid.ir/paper/107864/en

Vancouver: Copy

HOSSEINI SEYED ZEYNOLABEDIN, MASOOMI KARIMI MASOOMEH, JAFARI SANI MOSLEM, KAZEMI SHAHANDASHTI SADY, LAKOURAJ MANSOUR, GHAFARICHERATI RAMEZAN, TABAR MOLAHASAN AGHIL. DIAGNOSIS OF ANTI-NUCLEAR ANTIBODY USING CALF THYMUS COATED GLASS IN PATIENT WITH LUPUS. KNOWLEDGE AND HEALTH[Internet]. 2016;11(3 ):55-62. Available from: https://sid.ir/paper/107864/en

IEEE: Copy

SEYED ZEYNOLABEDIN HOSSEINI, MASOOMEH MASOOMI KARIMI, MOSLEM JAFARI SANI, SADY KAZEMI SHAHANDASHTI, MANSOUR LAKOURAJ, RAMEZAN GHAFARICHERATI, and AGHIL TABAR MOLAHASAN, “DIAGNOSIS OF ANTI-NUCLEAR ANTIBODY USING CALF THYMUS COATED GLASS IN PATIENT WITH LUPUS,” KNOWLEDGE AND HEALTH, vol. 11, no. 3 , pp. 55–62, 2016, [Online]. Available: https://sid.ir/paper/107864/en

Related Journal Papers