IN VITRO HEPATOCYTE-LIKE CELLS DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS

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GHADERI MASOUMEH; ALLAMEH ABDOLAMIR; SOLEIMANI MASOUD; FOROZANDE MOGHADAM MAHDI

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Journal: Journal of Knowledge & Health in Basic Medical Sciences Year:2012 | Volume:7 | Issue:2 Page(s): 50-55

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Title

IN VITRO HEPATOCYTE-LIKE CELLS DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS

Author(s)

GHADERI MASOUMEH | ALLAMEH ABDOLAMIR | SOLEIMANI MASOUD | FOROZANDE MOGHADAM MAHDI | Issue Writer Certificate

Keywords

UMBILICAL CORD BLOODQ3

MESENCHYMAL STEM CELLSQ3

DIFFERENTIATIONQ3

HEPATOCYTEQ3

Abstract

Introduction: Using stem cells for in vitro tissue regeneration is one of the most interesting fields of research. Liver regeneration is not an exceptional area. The main objective of this study was the production of HEPATOCYTE- like cells from the UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS (MSCs).Methods: Cells were isolated from UCB, upon a written informed consent obtained from donor mothers. The stem cells immunophenotype was analyzed using flow cytometry and multi-lineage DIFFERENTIATION potential was evaluated by means of in vitro DIFFERENTIATION assays. Finally, the expression of HEPATOCYTE-specific markers was verified using reverse transcription-polymerase chain reaction (RT-PCR).Results: The isolated UCB-MSCs had mesodermal DIFFERENTIATION capacity. If UCB-MSCs are cultured in appropriate conditions, they are able to induce hepatic-associated genes, such as albumin (Alb), a-fetoprotein (AFP), cytokeratins 18, 19 (CK18, CK19) and a-1 antitrypsin (AA). Moreover, UCB- MSCs produced albumin andα-fetoprotein upon in vitro induction of HEPATOCYTE DIFFERENTIATION.Conclusion: MESENCHYMAL STEM CELLS from human UMBILICAL CORD BLOOD are capable of tissue-specific commitment along hepatogenic lineage, proposing these cells as suitable tool for cell-based applications aimed at liver regeneration.

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APA: Copy

GHADERI, MASOUMEH, ALLAMEH, ABDOLAMIR, SOLEIMANI, MASOUD, & FOROZANDE MOGHADAM, MAHDI. (2012). IN VITRO HEPATOCYTE-LIKE CELLS DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS. KNOWLEDGE AND HEALTH, 7(2), 50-55. SID. https://sid.ir/paper/108098/en

Vancouver: Copy

GHADERI MASOUMEH, ALLAMEH ABDOLAMIR, SOLEIMANI MASOUD, FOROZANDE MOGHADAM MAHDI. IN VITRO HEPATOCYTE-LIKE CELLS DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS. KNOWLEDGE AND HEALTH[Internet]. 2012;7(2):50-55. Available from: https://sid.ir/paper/108098/en

IEEE: Copy

MASOUMEH GHADERI, ABDOLAMIR ALLAMEH, MASOUD SOLEIMANI, and MAHDI FOROZANDE MOGHADAM, “IN VITRO HEPATOCYTE-LIKE CELLS DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS,” KNOWLEDGE AND HEALTH, vol. 7, no. 2, pp. 50–55, 2012, [Online]. Available: https://sid.ir/paper/108098/en

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