INDUCTION OF HUMAN EMBRYONIC STEM CELLS INTO NEURONAL DIFFERENTIATION BY INCREASING CYCLIC ADENOSINE MONO PHOSPHATE

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BAHARVAND H.; HATAMI MARYAM; MASOUMI MOHAMMAD

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Journal: Physiology and Pharmacology Year:2006 | Volume:10 | Issue:1 Page(s): 11-19

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Title

INDUCTION OF HUMAN EMBRYONIC STEM CELLS INTO NEURONAL DIFFERENTIATION BY INCREASING CYCLIC ADENOSINE MONO PHOSPHATE

Author(s)

BAHARVAND H. | HATAMI MARYAM | MASOUMI MOHAMMAD | Issue Writer Certificate

Keywords

EMBRYONIC STEM CELLSQ3

CAMPQ4

NEUROGENESISQ3

Abstract

Introduction: To evaluate the CAMP -mediated IBMX (3-IsoButyle -1-Methyl Xanthin) and db-cAMP (dibutyryl CAMP) effects on differentiation of human EMBRYONIC STEM CELLS (hESCs) into nerve cells were the objectives of this study.Methods: We have used Royan H1 hESC- derived embryoid bodies with four treatment groups: six days treatment with IBMX (5×10 -4M) and db-cAMP (10 -9M) (referred to as CAMP), retinoic acid (RA, 10-6 M), IBMX + db-cAMP + RA, and control (no treatment). Immunocytochemistry was carried out for neural specific antibodies including b-Tubulin III, Microtubule Associated Protein 2 (MAP-2), Neurofilament Protein-Heavy chain (NF-H), Glial Fibrilary Acidic Protein (GFAP) and Synaptophysin as well as morphological studies. Semi quantitative RT-PCR was also used to evaluate gene expression involved in NEUROGENESIS.Results: In the 4+6+4 days the neuronal process were apparently observed. Immunocytochemical studies using nerve specific antibodies for proteins such as b- Tubulin III, MAP-2, NF-H, GFAP and Synaptophysin showed the presence of these neuronal and astrocyte markers in differentiated cells by CAMP. Evaluation of expression of genes involved in NEUROGENESIS showed that Hash1, Synaptophysin, β-Adrenergic Receptor and Acetylcholine Receptor- which were silent in embryoid bodies - switched on after treatment with CAMP and/or RA. Relative expression of nerve specific genes showed a significant enhancement in expression of Synaptophysin, NFM and b-Adrenergic Receptor during differentiation, which, with the enhancement in CAMP treated groups were more than those treated with RA and control (p<0.05).Conclusion: In conclusion, this study showed that CAMP could be a neurogenic agent for human EMBRYONIC STEM CELLS differentiation.

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APA: Copy

BAHARVAND, H., HATAMI, MARYAM, & MASOUMI, MOHAMMAD. (2006). INDUCTION OF HUMAN EMBRYONIC STEM CELLS INTO NEURONAL DIFFERENTIATION BY INCREASING CYCLIC ADENOSINE MONO PHOSPHATE. PHYSIOLOGY AND PHARMACOLOGY, 10(1), 11-19. SID. https://sid.ir/paper/74918/en

Vancouver: Copy

BAHARVAND H., HATAMI MARYAM, MASOUMI MOHAMMAD. INDUCTION OF HUMAN EMBRYONIC STEM CELLS INTO NEURONAL DIFFERENTIATION BY INCREASING CYCLIC ADENOSINE MONO PHOSPHATE. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2006;10(1):11-19. Available from: https://sid.ir/paper/74918/en

IEEE: Copy

H. BAHARVAND, MARYAM HATAMI, and MOHAMMAD MASOUMI, “INDUCTION OF HUMAN EMBRYONIC STEM CELLS INTO NEURONAL DIFFERENTIATION BY INCREASING CYCLIC ADENOSINE MONO PHOSPHATE,” PHYSIOLOGY AND PHARMACOLOGY, vol. 10, no. 1, pp. 11–19, 2006, [Online]. Available: https://sid.ir/paper/74918/en

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