THE EFFECT OF RETINOIC ACID HUMAN EMBRYONIC STEM CELLS WITH AND WITHOUT ROSETTE MORPHOLOGY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAHARVAND H.; HATAMI M.; ZARE N.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2004 | Volume:6 | Issue:22 Page(s): 103-109

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,163

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF RETINOIC ACID HUMAN EMBRYONIC STEM CELLS WITH AND WITHOUT ROSETTE MORPHOLOGY

Author(s)

BAHARVAND H. | HATAMI M. | ZARE N. | Issue Writer Certificate

Keywords

HUMAN EMBRYONIC STEM CELLQ3

ROSETTEQ3

DEFFERENTIATIONQ2

NEURONQ3

Abstract

Introduction: Human Embryonic Stem (hES) Cells are pluripotent cells derived form the inner cell mass of blastocyst. These unique cells have the potential to form virtually any cell type in the body and can be propagated in vitro indefinitely in an undifferentiated state. This study was initiated to assess the effect of retinoic acid (RA) on hES cells with and without ROSETTE morphology (neuroectodermal – like cells). Material and Methods: Embryoid bodies (EBs) were made from ES cell line (Royan H1) with and without rosset compartments. The EBs treated with retinoic acid (RA) (10 µM) for 20 days and the cultured on tissue culture plate individually containing NEURONal medium. Differentiated cells were evaluated by phase contrast microscopy and immunocytochemistry. Results: Differentiated cells were positive with the NEURON specific antibodies against microtubules associated protein 2 (a + b) (MAP – 2) , neurofilament protein (NP), NEURON – specific enolase (NSE), synaptophysin, and tubulin III. In presence of RA, more EBs were differentiated into NEURONs in both groups (in group with ROSETTE compartment, 73.2% v.s. 13.2%, P<0.01, and in group with non – ROSETTE compartment, 57.5% v.s. 19.5% P< 0.01). Moreover, RA in EBs with ROSETTE compartment increased neural morphology. Conclusion: These results showed RA induces hES into neurogenesis and ROSETTE compartments f hES have more potential for neurogenesis and these cells may be used in stem cell transplantation therapies for diseases in future.

Cites

No record.

References

Cite

APA: Copy

BAHARVAND, H., HATAMI, M., & ZARE, N.. (2004). THE EFFECT OF RETINOIC ACID HUMAN EMBRYONIC STEM CELLS WITH AND WITHOUT ROSETTE MORPHOLOGY. CELL JOURNAL (YAKHTEH), 6(22), 103-109. SID. https://sid.ir/paper/16431/en

Vancouver: Copy

BAHARVAND H., HATAMI M., ZARE N.. THE EFFECT OF RETINOIC ACID HUMAN EMBRYONIC STEM CELLS WITH AND WITHOUT ROSETTE MORPHOLOGY. CELL JOURNAL (YAKHTEH)[Internet]. 2004;6(22):103-109. Available from: https://sid.ir/paper/16431/en

IEEE: Copy

H. BAHARVAND, M. HATAMI, and N. ZARE, “THE EFFECT OF RETINOIC ACID HUMAN EMBRYONIC STEM CELLS WITH AND WITHOUT ROSETTE MORPHOLOGY,” CELL JOURNAL (YAKHTEH), vol. 6, no. 22, pp. 103–109, 2004, [Online]. Available: https://sid.ir/paper/16431/en

Related Journal Papers