Investigation on the activity of purified inulinase enzymes from fungal strains on the inulin extracted from chicory (Cichorium intybus) for production of fructose sweetener

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Abedi Faezeh; NAGHAVI NAFISEH SADAT; RANJBAR MONIREH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY Year:2022 | Volume:12 | Issue:2 Page(s): 1729-1739

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

Investigation on the activity of purified inulinase enzymes from fungal strains on the inulin extracted from chicory (Cichorium intybus) for production of fructose sweetener

Author(s)

Abedi Faezeh | NAGHAVI NAFISEH SADAT | RANJBAR MONIREH | Issue Writer Certificate

Keywords

Inulin

Chicory

Inulinase

Fungal strains

Enzyme purification

Abstract

Chicory is a rich plant source for the prebiotic inulin. The aim of this study was to evaluate the activity of purified inulinase enzyme from fungal strains on the inulin extracted from chicory. Inulin was extracted by soaking in water and precipitating in ethanol. Fungi were isolated from the soil around chicory root and screened for inulinase production along with fungal strains, Kluyveromyces marxianus PTCC 5006 and Aspergillus flavus PTCC 5304. The high inulinaseproducing fungal isolate was identified based on the sequencing of 18S rRNA gene. The enzyme was purified using ammonium sulfate-polyacrylamide and dialysis. The molecular weight of the protein was detected using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). One fungal isolate that showed high enzymatic activity (2233 U/ml) was obtained. This isolate was identified as the species Talaromyces moniliformis. Among the collection strains, Kluyveromyces marxianus had the highest inulinase production,while its inulinase activity was lower than that of Talaromyces moniliformis. The results from enzyme purification from Talaromyces moniliformis showed that the most specific enzyme activity (546. 6 U/mg) and the highest purification yield (62. 17%) were obtained by the precipitation in 55% ammonium sulfate. Dialysis of the protein resulted in the extraction of a protein with a reduced specific enzyme activity (188. 6 U/mg). The molecular weight of the purified protein was estimated at15 kDa. The fungal inulinase which purified from native resources in the present study can be used in food and pharmaceutical industries due to its high enzymatic activity.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

Abedi, Faezeh, NAGHAVI, NAFISEH SADAT, & RANJBAR, MONIREH. (2022). Investigation on the activity of purified inulinase enzymes from fungal strains on the inulin extracted from chicory (Cichorium intybus) for production of fructose sweetener. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, 12(2), 1729-1739. SID. https://sid.ir/paper/1116297/en

Vancouver: Copy

Abedi Faezeh, NAGHAVI NAFISEH SADAT, RANJBAR MONIREH. Investigation on the activity of purified inulinase enzymes from fungal strains on the inulin extracted from chicory (Cichorium intybus) for production of fructose sweetener. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY[Internet]. 2022;12(2):1729-1739. Available from: https://sid.ir/paper/1116297/en

IEEE: Copy

Faezeh Abedi, NAFISEH SADAT NAGHAVI, and MONIREH RANJBAR, “Investigation on the activity of purified inulinase enzymes from fungal strains on the inulin extracted from chicory (Cichorium intybus) for production of fructose sweetener,” INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, vol. 12, no. 2, pp. 1729–1739, 2022, [Online]. Available: https://sid.ir/paper/1116297/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops